通过放射自显影法研究铟-博来霉素复合物在小细胞肺癌细胞中的分布。

Distribution of 111In-bleomycin complex in small cell lung cancer cells by autoradiography.

作者信息

Hou D Y, Maruyama Y

机构信息

Department of Radiation Medicine, University of Kentucky Medical Center, Lexington.

出版信息

J Surg Oncol. 1992 Feb;49(2):93-7. doi: 10.1002/jso.2930490206.

DOI:10.1002/jso.2930490206

PMID:1371176

Abstract

The distribution of 111In-bleomycin Complex (111In-BLMC) in small cell lung cancer (SCLC) cells was studied by autoradiography. SCLC cells were exposed to 111In-BLMC and 111Indium chloride (111InCl3) for 1 hour, 3 hours, and 4 hours; washed with fresh medium; and spread on slides. The slides were smeared with NTB2 (NTB3) emulsion by wet or dry-mount technique and exposed 3 to 15 days. 111In-BLMC was found to localize in the cell nucleus and nuclear membrane (78.3%); 111InCl3 located mainly in the cytoplasm (52.3%). This distribution of labeled BLM may explain the mechanism of killing SCLC cells by 111In-BLMC.

摘要

通过放射自显影术研究了铟-博来霉素复合物（¹¹¹In-BLMC）在小细胞肺癌（SCLC）细胞中的分布。将SCLC细胞分别暴露于¹¹¹In-BLMC和氯化铟（¹¹¹InCl₃）1小时、3小时和4小时；用新鲜培养基洗涤；然后铺在载玻片上。通过湿装或干装技术将载玻片涂上NTB2（NTB3）乳剂，并曝光3至15天。发现¹¹¹In-BLMC定位于细胞核和核膜（78.3%）；¹¹¹InCl₃主要位于细胞质（52.3%）。标记博来霉素的这种分布可能解释了¹¹¹In-BLMC杀死SCLC细胞的机制。