Miossec C, Genevee C, Hercend T, Jitsukawa S
Laboratoire d'Hémato-immunologie, INSERM U333, Institut Gustave-Roussy, Villejuif, France.
Cell Immunol. 1992 Mar;140(1):173-83. doi: 10.1016/0008-8749(92)90185-r.
T lymphocytes express either the alpha/beta or the gamma/delta receptor (TCR) in a mutually exclusive fashion. Both structures are associated on the cell membrane with the CD3 proteins which are thought to transduce signals resulting from antigen recognition. The CD3 complex is present in both alpha/beta and gamma/delta cells and includes at least five proteins (designated gamma, delta, epsilon, zeta and eta). We have developed here a novel mAb, anti-CD3.TCR1, which immunoprecipitates the CD3 molecules from both alpha/beta and gamma/delta cells lysates following solubilization with Triton X-100. While the SDS-PAGE migration profile of the material recognized by either anti-CD3.TCR1 or anti-OKT3 are superimposable in both cell types, this mAb recognizes viable untreated gamma/delta T lymphocytes exclusively. These findings further support the view that molecular interactions within the TCR/CD3 protein complex are distinct in the two T lymphocyte populations.
T淋巴细胞以互斥的方式表达α/β或γ/δ受体（TCR）。这两种结构在细胞膜上均与CD3蛋白相关联，CD3蛋白被认为可转导抗原识别产生的信号。CD3复合物存在于α/β和γ/δ细胞中，至少包括五种蛋白质（分别命名为γ、δ、ε、ζ和η）。我们在此研发出一种新型单克隆抗体——抗CD3.TCR1，在用Triton X-100溶解后，它可从α/β和γ/δ细胞裂解物中免疫沉淀CD3分子。虽然抗CD3.TCR1或抗OKT3识别的物质在两种细胞类型中的SDS-PAGE迁移图谱可叠加，但这种单克隆抗体仅识别未处理的活γ/δ T淋巴细胞。这些发现进一步支持了以下观点：TCR/CD3蛋白复合物内的分子相互作用在两种T淋巴细胞群体中是不同的。
