Portoles P, Rojo J, Golby A, Bonneville M, Gromkowski S, Greenbaum L, Janeway C A, Murphy D B, Bottomly K
Department of Pathology, Yale University School of Medicine, New Haven, CT 06510.
J Immunol. 1989 Jun 15;142(12):4169-75.
The TCR is comprised of two variable chains that confer specificity, called alpha:beta or gamma:delta, physically associated with five different molecules that comprise the complex known as CD3. Antibodies to this complex are very useful, as they react with all T lymphocytes. A rat mAb to mouse CD3 has been prepared. It reacts with 100% of T cells in all mouse strains tested but with no other cell type. It binds to the CD3 epsilon chain. This antibody activates cloned T cell lines and normal T cells, provided suitable accessory cells and signals are present. This antibody detects a determinant similar to but not identical with those detected by two previously reported hamster anti-CD3 epsilon antibodies. This antibody fixes C efficiently, and it is thus useful for depletion of T cells from bulk populations. Activation of T cells by one of the three different anti-CD3 epsilon antibodies was inhibited by the Fab fragment of anti-CD4, similar to the effects of anti-CD4 Fab on two previously reported anti-TCR V region antibodies that bind a CD3 epsilon-associated epitope. This further defines a site involving TCR V regions and CD3 epsilon with which CD4 appears to associate during T cell activation.
T细胞受体（TCR）由两条赋予特异性的可变链组成，称为α:β或γ:δ，它们与构成称为CD3复合物的五种不同分子物理相连。针对该复合物的抗体非常有用，因为它们能与所有T淋巴细胞发生反应。已制备出一种针对小鼠CD3的大鼠单克隆抗体（mAb）。它能与所有测试小鼠品系中的100%的T细胞发生反应，但不与其他细胞类型发生反应。它与CD3 ε链结合。如果存在合适的辅助细胞和信号，该抗体可激活克隆的T细胞系和正常T细胞。该抗体检测到的决定簇与之前报道的两种仓鼠抗CD3 ε抗体检测到的决定簇相似但不完全相同。该抗体能有效地固定补体（C），因此可用于从大量细胞群体中清除T细胞。三种不同的抗CD3 ε抗体之一对T细胞的激活被抗CD4的Fab片段所抑制，这与抗CD4 Fab对之前报道的两种结合CD3 ε相关表位的抗TCR V区抗体的作用相似。这进一步确定了一个涉及TCR V区和CD3 ε的位点，在T细胞激活过程中CD4似乎与该位点相关联。
