Tsonis P A, Mescher A L, Del Rio-Tsonis K
Department of Biology, University of Dayton, OH 45469-2320.
Biochem J. 1992 Feb 1;281 ( Pt 3)(Pt 3):665-8. doi: 10.1042/bj2810665.
Protein synthesis has been studied by two-dimensional PAGE during the early limb regeneration in the adult newt. Quantitative and statistical analyses have provided unique information on overall patterns of protein synthesis as well as on specific protein synthesis during formation of the blastema. Furthermore, from the patterns in the two-dimensional gels and their quantification, a particular protein has been selected and sequenced. Partial sequencing revealed sequence similarities to Xenopus type I keratin B2. Expression of this keratin is 10-fold greater in the blastema than in the intact limb. The implications of keratin expression by the blastema cells are discussed.
在成年蝾螈早期肢体再生过程中，通过双向聚丙烯酰胺凝胶电泳对蛋白质合成进行了研究。定量和统计分析提供了有关蛋白质合成总体模式以及胚基形成过程中特定蛋白质合成的独特信息。此外，根据双向凝胶中的模式及其定量结果，选择了一种特定蛋白质并进行了测序。部分测序显示其与非洲爪蟾I型角蛋白B2有序列相似性。这种角蛋白在胚基中的表达比在完整肢体中高10倍。本文讨论了胚基细胞表达角蛋白的意义。
