在含有或不含十二烷基硫酸钠（SDS）的凝胶中对蛋白质进行咪唑-SDS-锌反向染色以及对单个未修饰的电转印蛋白质进行微测序
Imidazole-SDS-Zn reverse staining of proteins in gels containing or not SDS and microsequence of individual unmodified electroblotted proteins.
作者信息
Ortiz M L, Calero M, Fernandez Patron C, Patron C F, Castellanos L, Mendez E
机构信息
Servicio de Endocrinologia, Hospital Ramon y Cajal, Madrid, Spain.
出版信息
FEBS Lett. 1992 Jan 27;296(3):300-4. doi: 10.1016/0014-5793(92)80309-5.
A reverse staining procedure is described for the detection of proteins in acrylamide and agarose gels with and without SDS. Protein detection occurs a few minutes after electrophoresis. The sensitivity on acrylamide gels is higher than that of Coomassie blue staining either on acrylamide gels or on electrotransferred membranes. Sequencing of protein bands only detected by reverse staining on the gel and not by Coomassie blue is demonstrated.
本文描述了一种用于检测含或不含SDS的丙烯酰胺凝胶和琼脂糖凝胶中蛋白质的反向染色方法。蛋白质检测在电泳后几分钟内即可完成。丙烯酰胺凝胶上的灵敏度高于考马斯亮蓝染色在丙烯酰胺凝胶或电转膜上的灵敏度。展示了仅通过凝胶上的反向染色而非考马斯亮蓝染色检测到的蛋白质条带的测序结果。
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