Górnicka-Michalska Ewa, Kowalski Andrzej, Pałyga Jan
Department of Biochemistry and Genetics, Institute of Biology, Jan Kochanowski University, ul. Świętokrzyska 15, 25-406, Kielce, Poland.
Cell Mol Biol Lett. 2014 Mar;19(1):116-25. doi: 10.2478/s11658-014-0182-8. Epub 2014 Feb 6.
Two isoforms of the erythrocyte histone H1.a were identified in two conservative flocks of Rhode Island Red chickens and six conservative flocks of ducks. The H1.a1 and H1.a2 isoforms formed three phenotypes (a1, a2 and a1a2) and were electrophoretically similar in the two species. The frequency of phenotype and histone H1.a allele occurrence varied within the genetic groups of birds, but the relatively rare allele a(2) was only detected in chicken and duck strains with colored feathers. Using mass spectrometry, we established that the difference between the measured masses of the duck H1.a isoforms was 156 Da. Since this value corresponds to the mass of the arginine residue alone or to the combined mass of the valine and glycine residues, we believe that the polymorphism of duck histone H1.a might have originated from sequence variation. A mass difference of 1 Da observed between chicken H1.a isoforms corresponded well to the previously detected Glu/Lys substitution (0.9414 Da) at position 117.
在两群保守的罗德岛红鸡和六群保守的鸭子中鉴定出了红细胞组蛋白H1.a的两种亚型。H1.a1和H1.a2亚型形成了三种表型（a1、a2和a1a2），并且在这两个物种中电泳结果相似。表型频率和组蛋白H1.a等位基因出现频率在鸟类的遗传群体中有所不同，但相对罕见的等位基因a(2)仅在有彩色羽毛的鸡和鸭品系中被检测到。通过质谱分析，我们确定鸭H1.a亚型的测量质量之间的差异为156 Da。由于该值仅对应精氨酸残基的质量或缬氨酸和甘氨酸残基的组合质量，我们认为鸭组蛋白H1.a的多态性可能源于序列变异。在鸡H1.a亚型之间观察到的1 Da质量差异与先前在第117位检测到的Glu/Lys取代（0.9414 Da）非常吻合。
