Koller M E, Reed R K
Department of Anesthesiology, University of Bergen, Norway.
J Appl Physiol (1985). 1992 Jan;72(1):53-7. doi: 10.1152/jappl.1992.72.1.53.
This study shows that, in rat trachea, dextran anaphylaxis is associated with increased negativity of interstitial fluid pressure (Pif) as measured with sharpened glass capillaries (tip diameter 3-7 microns) connected to a servo-controlled counterpressure system. Experiments were carried out in pentobarbital-anesthetized Wistar-Møller rats. Pif in the control situation was -2.5 +/- 0.38 (SD) mmHg. The mean pressure in animals killed 2 min after initiation of the anaphylactic reaction by injection of 1 ml of 10% Dextran 70 in 0.9% NaCl was -10.3 +/- 2.6 mmHg. In another experimental series, interstitial fluid volume was measured after dextran administration but without inducing circulatory arrest. Interstitial fluid volume increased from 0.94 +/- 0.16 to 1.56 +/- 0.42 ml/g dry wt after 10 min to 1.57 +/- 0.30 and 1.10 +/- 0.27 ml/g dry wt after 30 and 60 min, respectively. The increased negativity in Pif in tracheal mucosa in the early phase of dextran anaphylaxis will markedly increase the transcapillary net filtration pressure in the initial phase of edema development.
本研究表明，在大鼠气管中，右旋糖酐过敏反应与间质液压力（Pif）的负值增加有关，该压力通过连接到伺服控制反压系统的尖锐玻璃毛细管（尖端直径3 - 7微米）进行测量。实验在戊巴比妥麻醉的Wistar - Møller大鼠身上进行。对照情况下的Pif为 -2.5 +/- 0.38（标准差）mmHg。在通过在0.9%氯化钠中注射1 ml 10%右旋糖酐70引发过敏反应2分钟后处死的动物中，平均压力为 -10.3 +/- 2.6 mmHg。在另一个实验系列中，在给予右旋糖酐后测量间质液体积，但不诱导循环停止。间质液体积在10分钟后从0.94 +/- 0.16增加到1.56 +/- 0.42 ml/g干重，在30分钟和60分钟后分别增加到1.57 +/- 0.30和1.10 +/- 0.27 ml/g干重。右旋糖酐过敏反应早期气管黏膜中Pif负值的增加将在水肿发展的初始阶段显著增加跨毛细血管净滤过压。
