HOPWOOD D A, GLAUERT A M
J Biophys Biochem Cytol. 1960 Dec;8(3):813-23. doi: 10.1083/jcb.8.3.813.
The chromatinic material of the blue-green alga Anabaena cylindrica has complex configurations in the central regions of the cells. The distribution of the chromatin within the cells varies in different filaments, probably in response to variations in the disposition of other cellular components. In electron micrographs of thin sections of organisms fixed by the method of Kellenberger, Ryter, and Séchaud (1958) the centroplasm contains fibrillar and possibly granular components which can be identified as the nuclear material by comparison with stained preparations viewed in the light microscope. The fibrils in the nuclear regions have diameters in the range of 5 to 7 mmicro and are embedded in a matrix of lower density. The nuclear regions are not greatly different from the cytoplasm in their electron density. Reducing the calcium content of the fixative results in coagulation of the fibrils to form coarser structures. The significance of the observations is discussed in relation to observations on the fine structure of other classes of algae and of bacteria.
蓝绿藻圆柱鱼腥藻的染色质物质在细胞的中央区域具有复杂的构型。细胞内染色质的分布在不同的丝状体中有所不同，这可能是对其他细胞成分排列变化的响应。在用凯伦伯格、赖特和塞肖德（1958年）的方法固定的生物体薄切片的电子显微照片中，中央质含有纤维状以及可能的颗粒状成分，通过与光学显微镜下观察的染色制剂进行比较，可以将其鉴定为核物质。核区域中的纤维直径在5至7微米范围内，并嵌入密度较低的基质中。核区域的电子密度与细胞质没有太大差异。降低固定剂中的钙含量会导致纤维凝结形成更粗的结构。结合对其他藻类和细菌类群精细结构的观察，对这些观察结果的意义进行了讨论。
