Tobiasch E, Schweizer J, Winter H
Institute of Biochemistry, German Cancer Research Center, Heidelberg.
Mol Biol Rep. 1992 Feb;16(1):39-47. doi: 10.1007/BF00788752.
We present here a 1770 bp-long cDNA which encodes a murine type II keratin. Sequence comparisons of the keratin with those of various type II keratins expressed in mouse epidermis and internal stratified epithelia reveal that the new keratin is unrelated to epithelial keratins. Rather the structural organization of its amino- and carboxyterminal domains and the high content of cysteine and proline residues in these regions suggest that the keratin represents a murine type II hair keratin. This assumption was confirmed by in situ hybridization which localized the mRNA of the keratin in upper cells of the hair cortex and in suprabasal cells of the central core unit of filiform papillae of the tongue. Hybrid selection analyses revealed that the keratin has a molecular weight of 58 kD. It remains to be seen whether the keratin corresponds to MHb 3 or MHb 4.
我们在此展示一个长度为1770 bp的cDNA，其编码一种小鼠II型角蛋白。将该角蛋白与在小鼠表皮和内部复层上皮中表达的各种II型角蛋白进行序列比较，结果显示这种新的角蛋白与上皮角蛋白无关。相反，其氨基末端和羧基末端结构域的结构组织以及这些区域中高含量的半胱氨酸和脯氨酸残基表明，该角蛋白代表一种小鼠II型毛发角蛋白。原位杂交证实了这一假设，该杂交将角蛋白的mRNA定位在毛皮质的上层细胞以及舌丝状乳头中央核心单元的基底层以上细胞中。杂交选择分析表明，该角蛋白的分子量为58 kD。它是否对应于MHb 3或MHb 4还有待观察。
