Diaz de Arce H, Artursson K, L'Haridon R, Perers A, La Bonnardiere C, Alm G V
Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, Uppsala.
Vet Immunol Immunopathol. 1992 Jan 31;30(4):319-27. doi: 10.1016/0165-2427(92)90102-v.
Two murine monoclonal antibodies (mAbs) directed against different epitopes on recombinant porcine interferon-alpha (IFN-alpha) were selected and used to construct a two-site ELISA. This ELISA, when performed in a one-step version, detected about 0.5 units ml-1 of IFN-alpha and showed similar sensitivity but better precision than a cytopathic effect inhibition bioassay. Estimates of IFN-alpha in tissue culture medium by the two assays correlated well. In contrast, one or several factors in porcine serum reduced the sensitivity of the ELISA. Measurements of IFN-alpha in porcine serum was, however, possible in a two-step version of the ELISA, with a sensitivity of about 1 unit IFN-alpha ml-1. Results of ELISA and bioassay agreed, except that the ELISA possibly produced false positive results in two out of a total of 91 sera negative in the bioassay. In addition, one of 23 sera positive in the bioassay was negative in the ELISA.
选择了两种针对重组猪α干扰素（IFN-α）不同表位的鼠单克隆抗体（mAb），并用于构建双位点酶联免疫吸附测定（ELISA）。这种ELISA在一步法中进行时，可检测到约0.5单位/毫升的IFN-α，与细胞病变效应抑制生物测定法相比，灵敏度相似，但精密度更高。两种测定法对组织培养基中IFN-α的估计结果相关性良好。相比之下，猪血清中的一种或几种因素会降低ELISA的灵敏度。不过，在ELISA的两步法中可以测定猪血清中的IFN-α，灵敏度约为1单位IFN-α/毫升。ELISA和生物测定法的结果一致，但ELISA可能在总共91份生物测定法阴性的血清中产生了2例假阳性结果。此外，在生物测定法中呈阳性的23份血清中有1份在ELISA中呈阴性。
