Garlisi C G, Mastro A M
Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Vet Immunol Immunopathol. 1992 Jan 31;30(4):359-72. doi: 10.1016/0165-2427(92)90105-y.
Interleukin-2 (IL-2) is a lymphokine which, upon binding to its receptor, leads to the proliferation and differentiation of T-cells (helper, suppressor, and cytotoxic) and B-cells. While human and murine IL-2 have been extensively studied, less is known about bovine IL-2. In order to understand the induction of bovine IL-2 at the molecular level, we have examined IL-2 mRNA induction. The dose-responses and time courses of the production of IL-2 mRNA in response to Concanavalin A (ConA), 12-O-tetradecanoylphorbol-13-acetate (TPA), and ionomycin in lymph node lymphocytes (LNC) were determined. We found that high levels of IL-2 mRNA were produced in response to 1 microgram ml-1 ConA plus 10(-8) M TPA, but that even higher levels were produced in response to 1 microM ionomycin plus 10(-8) M TPA. We also found that LNC stimulated with ConA displayed two phases of IL-2 mRNA production, one occurring approximately 2-4 h after stimulation and one occurring approximately 10 h after stimulation. However, in the presence of ConA plus TPA or ionomycin plus TPA the response was monophasic. IL-2 mRNA was detected within 2 h of addition of ConA plus TPA (the earliest time examined), reached maximum levels within 6 h, and declined to low levels after 12 h. IL-2 mRNA from LNC incubated with ionomycin plus TPA appeared within 2 h, and reached maximum levels at about 9 h. In contrast to the decrease seen after 12 h with ConA plus TPA, IL-2 mRNA from these cells remained high for 18 h and declined to low levels after 24 h.
白细胞介素-2（IL-2）是一种淋巴因子，它与受体结合后，会导致T细胞（辅助性T细胞、抑制性T细胞和细胞毒性T细胞）和B细胞的增殖与分化。虽然人和小鼠的IL-2已得到广泛研究，但关于牛IL-2的了解却较少。为了在分子水平上理解牛IL-2的诱导过程，我们检测了IL-2 mRNA的诱导情况。测定了淋巴结淋巴细胞（LNC）对刀豆球蛋白A（ConA）、12-O-十四烷酰佛波醇-13-乙酸酯（TPA）和离子霉素产生IL-2 mRNA的剂量反应和时间进程。我们发现，1微克/毫升ConA加10^(-8) M TPA可诱导产生高水平的IL-2 mRNA，但1微摩尔离子霉素加10^(-8) M TPA诱导产生的水平更高。我们还发现，用ConA刺激的LNC表现出两个阶段的IL-2 mRNA产生，一个阶段大约在刺激后2至4小时出现，另一个阶段大约在刺激后10小时出现。然而，在ConA加TPA或离子霉素加TPA存在的情况下，反应是单相的。添加ConA加TPA后2小时内（检测的最早时间）即可检测到IL-2 mRNA，6小时内达到最高水平，12小时后降至低水平。用离子霉素加TPA孵育的LNC中的IL-2 mRNA在2小时内出现，约9小时达到最高水平。与ConA加TPA处理12小时后出现的下降情况不同，这些细胞的IL-2 mRNA在18小时内保持高水平，24小时后降至低水平。
