HIV逆转录酶的核糖核酸酶H活性仅局限于二聚体形式。
RNase H activity of HIV reverse transcriptases is confined exclusively to the dimeric forms.
作者信息
Restle T, Müller B, Goody R S
机构信息
Abteilung Biophysik, Max-Planck-Institut für medizinische Forschung, Heidelberg, Germany.
出版信息
FEBS Lett. 1992 Mar 23;300(1):97-100. doi: 10.1016/0014-5793(92)80172-d.
PMID:1372272
Abstract
A method for the rapid preparation of a defined substrate to monitor RNase H activity has been developed. Using this substrate, we have investigated the RNase H activities of the different forms of recombinant HIV-1 and HIV-2 reverse transcriptase (RT) in detail. As we report here, RNase H activity is associated only with the dimeric forms (p51/p66 or p66/p66) of the enzymes.
摘要
已经开发出一种快速制备用于监测核糖核酸酶H（RNase H）活性的特定底物的方法。使用该底物，我们详细研究了不同形式的重组HIV-1和HIV-2逆转录酶（RT）的RNase H活性。正如我们在此报道的，RNase H活性仅与这些酶的二聚体形式（p51/p66或p66/p66）相关。
Zotero 插件