Izumi R, Shimizu K, Kiriyama M, Hashimoto T, Urade M, Yagi M, Mizukami Y, Nonomura A, Miyazaki I
Department of Surgery 2, Kanazawa University, Japan.
J Surg Oncol. 1992 Mar;49(3):151-5. doi: 10.1002/jso.2930490305.
Immunohistochemistry using the avidin-biotin-peroxidase complex method was performed to study the production of alpha-fetoprotein (AFP) in hepatocellular carcinoma (HCC) tissue specimens which were obtained surgically. The relationship between staining for AFP and serum AFP levels or pathological findings was examined. The prognosis of the patients with HCC who underwent curative hepatic resections was studied with respect to the staining for AFP in their tumors. The mean serum AFP level in patients with positive AFP staining was significantly higher than in those with negative AFP staining. No significant relationship was found between AFP positivity and tumor size, tumor encapsulation, degree of vascular invasion, or the histological differentiation grade of the tumor. The patients with AFP-positive carcinomas had a poorer prognosis than did those with AFP-negative carcinomas (5-year survival rate of AFP-positive and negative groups were 26.7% and 56.5%, respectively.
采用抗生物素蛋白-生物素-过氧化物酶复合物法进行免疫组织化学检测，以研究手术获取的肝细胞癌（HCC）组织标本中甲胎蛋白（AFP）的产生情况。检测了AFP染色与血清AFP水平或病理结果之间的关系。针对接受根治性肝切除术的HCC患者，研究了其肿瘤中AFP染色与预后的关系。AFP染色阳性患者的平均血清AFP水平显著高于染色阴性患者。未发现AFP阳性与肿瘤大小、肿瘤包膜、血管侵犯程度或肿瘤组织学分化等级之间存在显著关系。AFP阳性癌患者的预后比AFP阴性癌患者差（AFP阳性和阴性组的5年生存率分别为26.7%和56.5%）。
