Zlatopolsky A D, Chubukina A N, Berman A E
Institute of Biological and Medical Chemistry, USSR Academy of Medical Sciences, Moscow.
Biochem Biophys Res Commun. 1992 Mar 16;183(2):383-9. doi: 10.1016/0006-291x(92)90492-4.
The proliferation promoting activity of various proteolytic fragments of human plasma fibronectin was assayed. Study of this activity in fragments, purified by affinity chromatography, has shown that only heparin-binding fragments were capable of promoting fibroblast proliferation while gelatin- and fibrin-binding fragments were not. Heparin-binding fragments with high affinity for heparin were characterized by high activity levels while those with low heparin affinity were inactive. Heparin-binding fragments with the highest proliferation promoting activity contained the cell-binding domain and were virtually devoid of the hep2, hep1 and gelatin-binding domains.
对人血浆纤连蛋白各种蛋白水解片段的增殖促进活性进行了测定。对通过亲和层析纯化的片段中的这种活性进行研究表明，只有肝素结合片段能够促进成纤维细胞增殖，而明胶结合片段和纤维蛋白结合片段则不能。对肝素具有高亲和力的肝素结合片段具有高活性水平，而对肝素具有低亲和力的片段则无活性。具有最高增殖促进活性的肝素结合片段包含细胞结合结构域，并且实际上没有hep2、hep1和明胶结合结构域。
