Dunne W M, Burd E M
Department of Pathology, Baylor College of Medicine, Texas Children's Hospital, Houston 77030.
J Appl Bacteriol. 1993 Apr;74(4):411-6. doi: 10.1111/j.1365-2672.1993.tb05147.x.
The adhesion of five strains of slime-positive Staphylococcus epidermidis to plastic microwells was significantly diminished (P < 0.005) in a concentration-dependent fashion when wells were previously coated with increasing concentrations (1.6-13.1 micrograms cm-2) of human fibronectin (FN). The adhesion of four of five strains was significantly reduced when wells were coated with 3.2 micrograms cm-2 of FN and at concentrations > or = 6.5 micrograms cm-2 the adhesion of all slime-positive strains was significantly reduced. The coating of microwells with chymotryptic fragments of FN containing the heparin-binding, gelatin-binding, or cell-binding domains also reduced bacterial adhesion but none of the fragments exceeded the anti-adhesive activity of intact FN. A comparison of FN-coated or albumin-coated microwells showed that both proteins caused a significant reduction in the adhesion of test strains to plastic but that the anti-adhesive activity of FN was greater than albumin at all concentrations tested. The adhesion of the slime-negative phase variant of one of the test strains to plastic was neither enhanced nor reduced by FN coating indicating that the production of an exopolysaccharide by Staph. epidermidis influences interactions with protein-coated surfaces. These results support the contention that FN does not mediate the adhesion of all strains of Staph. epidermidis to plastic surfaces.
当塑料微孔预先用浓度不断增加（1.6 - 13.1微克/平方厘米）的人纤连蛋白（FN）包被时，五株黏液阳性表皮葡萄球菌对塑料微孔的黏附力以浓度依赖方式显著降低（P < 0.005）。当微孔用3.2微克/平方厘米的FN包被时，五株中的四株黏附力显著降低，而当浓度≥6.5微克/平方厘米时，所有黏液阳性菌株的黏附力均显著降低。用含有肝素结合、明胶结合或细胞结合结构域的FN胰凝乳蛋白酶片段包被微孔也能降低细菌黏附，但没有一个片段的抗黏附活性超过完整FN。对用FN包被或白蛋白包被的微孔进行比较表明，两种蛋白质都能显著降低测试菌株对塑料的黏附，但在所有测试浓度下，FN的抗黏附活性都大于白蛋白。FN包被对测试菌株之一的黏液阴性相变变体与塑料的黏附没有增强或降低作用，这表明表皮葡萄球菌产生的胞外多糖会影响与蛋白包被表面的相互作用。这些结果支持了FN并不介导所有表皮葡萄球菌菌株对塑料表面黏附的观点。
