Larsen P J, Saermark T, Mau S E
Institute of Medical Anatomy B, University of Copenhagen, Denmark.
J Histochem Cytochem. 1992 Apr;40(4):487-93. doi: 10.1177/40.4.1372633.
Several lines of anatomic, biochemical, and pharmacological evidence suggest that the neuropeptide substance P has a direct action on cells of the anterior pituitary lobe via a specific neurokinin-1 receptor. In the present study we confirmed this association by combining Bolton-Hunter iodinated substance P-receptor autoradiography with immunocytochemistry on cultured anterior pituitary cells. Radiolabeled substance P was bound to living cell cultures at 0 degrees C, and after a brief wash the cultures were fixed and processed immunocytochemically for prolactin and luteinizing hormone. A large proportion of cultured anterior pituitary cells possessed substance P binding sites. When receptor autoradiography was combined with immunocytochemistry, it was evident that both prolactin- and luteinizing hormone-immunoreactive cells were labeled with radiolabeled substance P. However, a small proportion of the radioligand-labeled cells were not stained by the immunocytochemical procedure, suggesting that additional cell types possess substance P receptors. The present study presents morphological evidence that substance P binds to prolactin- and luteinizing hormone-containing cells of the anterior pituitary lobe. Therefore, it is likely that substance P has a direct action on mammotrophs and gonadotrophs.
多条解剖学、生物化学及药理学证据表明，神经肽P物质通过特定的神经激肽-1受体对垂体前叶细胞具有直接作用。在本研究中，我们通过将博尔顿-亨特碘化P物质受体放射自显影与培养的垂体前叶细胞免疫细胞化学相结合，证实了这种关联。放射性标记的P物质在0℃下与活细胞培养物结合，短暂冲洗后，将培养物固定并进行催乳素和促黄体生成素的免疫细胞化学处理。大部分培养的垂体前叶细胞具有P物质结合位点。当放射自显影与免疫细胞化学相结合时，很明显催乳素和促黄体生成素免疫反应性细胞均被放射性标记的P物质标记。然而，一小部分放射性配体标记的细胞未被免疫细胞化学方法染色，这表明其他细胞类型也具有P物质受体。本研究提供了形态学证据，表明P物质与垂体前叶含催乳素和促黄体生成素的细胞结合。因此，P物质很可能对乳腺促性腺激素细胞和促性腺激素细胞有直接作用。
