Upton S J, Tilley M
Division of Biology, Kansas State University, Manhattan 66506.
J Parasitol. 1992 Apr;78(2):329-33.
A technique was developed to examine the effects of exogenous substances on apicomplexan sporozoite motility in vitro. Sporozoites of Eimeria nieschulzi were placed in the top compartments of blind well chemotactic chambers and separated from potential chemoattractant/chemokinetic agents by 8.0-microns pore size Millipore filters. After 3-hr incubations, the number of sporozoites migrating through the filters was assessed using a hemacytometer. Results revealed that several substances, especially albumin and fetuin, enhanced motility of coccidial sporozoites. Cell culture assays supported these data, with higher numbers of parasites found in cultures supplemented with albumin and/or fetuin.
开发了一种技术，用于在体外检测外源性物质对顶复门孢子虫动子运动的影响。将尼氏艾美耳球虫的子孢子置于盲孔趋化室的顶部隔室中，并通过孔径为8.0微米的密理博滤膜与潜在的化学引诱剂/化学促动剂分离。孵育3小时后，使用血细胞计数器评估穿过滤膜的子孢子数量。结果显示，几种物质，尤其是白蛋白和胎球蛋白，增强了球虫亚目的子孢子的运动性。细胞培养试验支持了这些数据，在添加了白蛋白和/或胎球蛋白的培养物中发现了更多的寄生虫。
