Strout R G, Alroy J, Lukacs N W, Ward H D, Pereira M E
Department of Animal and Nutritional Sciences, University of New Hampshire, Durham 03824.
J Parasitol. 1994 Dec;80(6):946-51.
Avian coccidiosis caused by Eimeria species is characterized by rather specific site infections of the intestine. We used hemagglutination and hemagglutination inhibition assays on various developmental stages of Eimeria tenella and on sporozoites of Eimeria acervulina and Eimeria maxima to assay for parasite lectins. Various monosaccharides, polysaccharides, and glycoproteins were used to demonstrate differences in sugar specificity of the lectins between these species. Surface lectins were found on the primary infective stage, i.e., sporozoites, but not on merozoites or unsporulated oocysts. Also, there were differences in the specificities of the various sugar lectins among the different parasite species. Furthermore, there was a dose-dependent reduction of infection of tissue culture cells by sporozoites of E. tenella that were continuously exposed to fetuin, 1 of the specific inhibitors of the lectin. The results of our study are unique in that in 3 species of avian Eimeria all have a lectin on their sporozoites, but the lectins for each species have different sugar specificities. We hypothesize that these lectins found on the surface of the sporozoites may play a role in determining the site of infection within the intestine of the host.
由艾美耳球虫引起的禽球虫病的特征是肠道有相当特定部位的感染。我们对柔嫩艾美耳球虫的各个发育阶段以及堆型艾美耳球虫和巨型艾美耳球虫的子孢子进行了血凝和血凝抑制试验，以检测寄生虫凝集素。使用了各种单糖、多糖和糖蛋白来证明这些物种之间凝集素糖特异性的差异。在主要感染阶段即子孢子上发现了表面凝集素，但在裂殖子或未孢子化的卵囊上未发现。此外，不同寄生虫物种之间各种糖凝集素的特异性也存在差异。此外，持续暴露于凝集素的特异性抑制剂之一胎球蛋白后，柔嫩艾美耳球虫子孢子对组织培养细胞的感染呈剂量依赖性降低。我们的研究结果独特之处在于，在三种禽艾美耳球虫中，所有虫种的子孢子上都有凝集素，但每个虫种的凝集素具有不同的糖特异性。我们推测在子孢子表面发现的这些凝集素可能在确定宿主肠道内的感染部位中起作用。
