Caccone A, Gleason J M, Powell J R
Department of Biology, Yale University, New Haven, CT 06511.
J Mol Evol. 1992 Feb;34(2):130-40. doi: 10.1007/BF00182390.
We have performed DNA-DNA hybridization experiments among several species of Drosophila using the evolutionarily conserved portion of the genome representing sequences coding for amino acids of proteins. This was done by using as tracer, radioactively labeled complementary DNA that was reverse transcribed from adult mRNA. We show that this procedure extends phylogenetically the distance over which the technique can be applied to fast-evolving groups such as Drosophila. The major phylogenetic conclusions are (1) the subgenus Sophophora is a monophyletic lineage; (2) within Sophophora the melanogaster subgroup is closer to the obscura group than either group is to the willistoni group; (3) the subgenus Drosophila is complex with most major lineages originating deep in the phylogeny; the subgenus may not be monophyletic; (4) as with most groups classically placed in Drosophila, the Hawaiian Drosophila originate early, supporting the notion that this lineage is older than the extant islands; and (5) the virilis/repleta lineage is monophyletic within Drosophila.
我们利用基因组中代表蛋白质氨基酸编码序列的进化保守部分，在几种果蝇物种间进行了DNA-DNA杂交实验。这是通过使用放射性标记的互补DNA作为示踪剂来完成的，该互补DNA是从成年果蝇的mRNA反转录而来。我们表明，该方法在系统发育上扩展了该技术可应用于快速进化群体（例如果蝇）的距离范围。主要的系统发育结论如下：（1）Sophophora亚属是一个单系谱系；（2）在Sophophora亚属中，黑腹果蝇亚组与obscura组的关系比这两个组与willistoni组的关系更近；（3）果蝇亚属很复杂，大多数主要谱系起源于系统发育的深处；该亚属可能不是单系的；（4）与大多数传统上归为果蝇属的类群一样，夏威夷果蝇起源较早，这支持了该谱系比现存岛屿更古老的观点；（5）virilis/repleta谱系在果蝇属内是单系的。
