Guerrero C, Mateos L M, Malumbres M, Martín J F
Department of Ecology, Genetics and Microbiology, Faculty of Biology, University of León, Spain.
Appl Microbiol Biotechnol. 1992 Mar;36(6):759-62. doi: 10.1007/BF00172189.
Corynebacteria are highly sensitive to the glycopeptide antibiotic bleomycin. The bleomycin resistance gene of transposon Tn5 is expressed very efficiently in Brevibacterium lactofermentum. This gene constitutes an excellent marker for selection of transformants of corynebacteria. The bleomycin resistance gene is expressed from the same promoter as the neomycin resistance gene, which is already used as marker in many vectors of corynebacteria. The promoter of the neo-ble cluster is expressed in a variety of Gram-negative and Gram-positive microorganisms and eucaryotic organisms.
棒状杆菌对糖肽类抗生素博来霉素高度敏感。转座子Tn5的博来霉素抗性基因在乳酸发酵短杆菌中表达效率很高。该基因构成了用于筛选棒状杆菌转化体的优良标记。博来霉素抗性基因与新霉素抗性基因由同一个启动子表达，新霉素抗性基因已在许多棒状杆菌载体中用作标记。neo-ble基因簇的启动子在多种革兰氏阴性和革兰氏阳性微生物以及真核生物中均有表达。
