用于乳酸发酵短杆菌的穿梭载体系统。
A shuttle vector system for Brevibacterium lactofermentum.
作者信息
Yeh P, Oreglia J, Prévots F, Sicard A M
出版信息
Gene. 1986;47(2-3):301-6. doi: 10.1016/0378-1119(86)90074-0.
PMID:3549456
Abstract
We have constructed a shuttle vector that replicates in Escherichia coli, Corynebacterium glutamicum and Brevibacterium lactofermentum, by fusion of a 4.4-kb cryptic plasmid isolated from B. lactofermentum and a derivative of pBR322. Resistance to erythromycin which is expressed in all three bacteria has been a useful selective marker. The frequency of homospecific transformation was 1.5 X 10(5) transformants/micrograms of hybrid plasmid DNA.
摘要
我们通过将从乳酸发酵短杆菌中分离出的4.4 kb隐蔽质粒与pBR322的衍生物融合，构建了一种穿梭载体，该载体可在大肠杆菌、谷氨酸棒杆菌和乳酸发酵短杆菌中复制。在所有三种细菌中均表达的对红霉素的抗性一直是一种有用的选择标记。同种特异性转化频率为1.5×10⁵个转化子/微克杂交质粒DNA。
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