猫心肌的体外研究。I. 乳头肌的细胞体积和细胞内浓度
Cat heart muscle in vitro. I. Cell volumes and intracellular concentrations in papillary muscle.
作者信息
PAGE E, SOLOMON A K
出版信息
J Gen Physiol. 1960 Nov;44(2):327-44. doi: 10.1085/jgp.44.2.327.
Abstract
Methods have been developed for the simultaneous determination of total water, inulin space, and K and Na content in muscles of 0.5 to 10 mg. wet weight. These methods have been used to define steady state conditions with respect to intracellular K concentration in papillary muscles from cat hearts perfused and contracting isometrically at 27-28 degrees C. and at 37-38 degrees C. Cell volumes and intracellular ionic concentrations have been followed as a function of the external K concentration and compared with values predicted on the basis of electroneutrality and osmotic equilibrium.
摘要
已开发出一些方法，可同时测定湿重为0.5至10毫克的肌肉中的总水量、菊粉分布容积以及钾和钠含量。这些方法已用于确定在27 - 28摄氏度和37 - 38摄氏度下等长灌注并收缩的猫心乳头肌细胞内钾浓度的稳态条件。已跟踪细胞体积和细胞内离子浓度随外部钾浓度的变化情况，并与基于电中性和渗透平衡预测的值进行比较。
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