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猫心肌的体外研究。I. 乳头肌的细胞体积和细胞内浓度

Cat heart muscle in vitro. I. Cell volumes and intracellular concentrations in papillary muscle.

作者信息

PAGE E, SOLOMON A K

出版信息

J Gen Physiol. 1960 Nov;44(2):327-44. doi: 10.1085/jgp.44.2.327.

DOI:10.1085/jgp.44.2.327
PMID:13732016
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2195100/
Abstract

Methods have been developed for the simultaneous determination of total water, inulin space, and K and Na content in muscles of 0.5 to 10 mg. wet weight. These methods have been used to define steady state conditions with respect to intracellular K concentration in papillary muscles from cat hearts perfused and contracting isometrically at 27-28 degrees C. and at 37-38 degrees C. Cell volumes and intracellular ionic concentrations have been followed as a function of the external K concentration and compared with values predicted on the basis of electroneutrality and osmotic equilibrium.

摘要

已开发出一些方法，可同时测定湿重为0.5至10毫克的肌肉中的总水量、菊粉分布容积以及钾和钠含量。这些方法已用于确定在27 - 28摄氏度和37 - 38摄氏度下等长灌注并收缩的猫心乳头肌细胞内钾浓度的稳态条件。已跟踪细胞体积和细胞内离子浓度随外部钾浓度的变化情况，并与基于电中性和渗透平衡预测的值进行比较。

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本文引用的文献

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The effect of sodium ions on the electrical activity of giant axon of the squid.钠离子对鱿鱼巨大轴突电活动的影响。
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A study of inotropic mechanisms in the papillary muscle preparation.乳头肌标本中变力机制的研究。
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