Dedieu J F, Ronco J, van der Werf S, Hogle J M, Henin Y, Girard M
Unité de Virologie Moléculaire (Centre National de la Recherche Scientifique UA 545), Institut Pasteur, Paris, France.
J Virol. 1992 May;66(5):3161-7. doi: 10.1128/JVI.66.5.3161-3167.1992.
Sequences from the principal neutralization domain of human immunodeficiency virus type 1 (HIV-1) strain LAI or RF have been expressed in antigenic site 1 of the capsid of the Sabin strain of poliovirus type 1. A number of the resulting chimeras were viable. Viable variants bearing mutations within the insertion site spontaneously arose from several nonviable chimeras. In general, these mutations result in a decrease in positive charge in the substituted antigenic site 1. Two of the chimeras were genetically stable and have been further characterized. Both chimeras were neutralized by various HIV-1 neutralizing antibodies. In rabbits, both chimeras produced high levels of antibodies which react with HIV-1 gp120/160 in immunoprecipitation and enzyme-linked immunosorbent assays. One of the chimeras (HIV-1LAI) produced a significant but weak HIV-1 neutralizing response.
来自1型人类免疫缺陷病毒（HIV-1）毒株LAI或RF主要中和结构域的序列已在1型脊髓灰质炎病毒Sabin株衣壳的抗原位点1中表达。许多所得嵌合体是可行的。在几个不可行的嵌合体中自发产生了在插入位点内带有突变的可行变体。一般来说，这些突变导致被取代的抗原位点1中的正电荷减少。其中两个嵌合体在遗传上是稳定的，并已得到进一步表征。两种嵌合体都被各种HIV-1中和抗体中和。在兔子中，两种嵌合体都产生了高水平的抗体，这些抗体在免疫沉淀和酶联免疫吸附试验中与HIV-1 gp120/160发生反应。其中一种嵌合体（HIV-1LAI）产生了显著但较弱的HIV-1中和反应。
