感染嵌合植物病毒后在植物中产生的抗原可针对狂犬病病毒和HIV-1进行免疫。
Antigens produced in plants by infection with chimeric plant viruses immunize against rabies virus and HIV-1.
作者信息
Yusibov V, Modelska A, Steplewski K, Agadjanyan M, Weiner D, Hooper D C, Koprowski H
机构信息
Biotechnology Foundation Laboratories, Thomas Jefferson University, 1020 Locust Street, Room 346 JAH, Philadelphia, PA 19107, USA.
出版信息
Proc Natl Acad Sci U S A. 1997 May 27;94(11):5784-8. doi: 10.1073/pnas.94.11.5784.
Abstract
The coat protein (CP) of alfalfa mosaic virus was used as a carrier molecule to express antigenic peptides from rabies virus and HIV. The antigens were separately cloned into the reading frame of alfalfa mosaic virus CP and placed under the control of the subgenomic promoter of tobacco mosaic virus CP in the 30BRz vector. The in vitro transcripts of recombinant virus with sequences encoding the antigenic peptides were synthesized from DNA constructs and used to inoculate tobacco plants. The plant-produced protein (virus particles) was purified and used for immunization of mice. Both antigens elicited specific virus-neutralizing antibodies in immunized mice.
摘要
苜蓿花叶病毒的外壳蛋白（CP）被用作载体分子来表达来自狂犬病病毒和HIV的抗原肽。这些抗原分别被克隆到苜蓿花叶病毒CP的阅读框中，并置于30BRz载体中烟草花叶病毒CP亚基因组启动子的控制之下。从DNA构建体合成了带有编码抗原肽序列的重组病毒的体外转录本，并用于接种烟草植株。对植物产生的蛋白（病毒颗粒）进行纯化，并用于免疫小鼠。两种抗原在免疫小鼠中均引发了特异性的病毒中和抗体。
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