Collins B W, Howard D R, Allen J W
Genetic Toxicology Division, U.S. Environmental Protection Agency, Research Triangle Park, NC.
Mutat Res. 1992 Apr;281(4):287-94. doi: 10.1016/0165-7992(92)90023-b.
The rodent spermatid micronucleus (MN) assay was used in conjunction with immunofluorescent techniques to distinguish kinetochores in MN following exposure of mice to X-radiation or acrylamide. After either treatment, modest increases in kinetochore-positive MN were observed. Spermatids which had been exposed during meiotic prophase to X-rays (400 cGy) had approximately 10-fold increases in MN compared to controls; up to 15% of the MN observed were kinetochore-positive. Following acrylamide treatment of meiotic prophase cells, there was a doubling of spermatid MN over baseline levels, approximately one-third of which were kinetochore-positive.
将啮齿动物精子细胞微核（MN）试验与免疫荧光技术结合使用，以区分小鼠经X射线或丙烯酰胺照射后微核中的动粒。两种处理后，均观察到动粒阳性微核有适度增加。在减数分裂前期暴露于X射线（400 cGy）的精子细胞，其微核数量相比对照组增加了约10倍；观察到的微核中高达15%为动粒阳性。用丙烯酰胺处理减数分裂前期细胞后，精子细胞微核数量比基线水平增加了一倍，其中约三分之一为动粒阳性。
