Allen J W, Collins B W, Setzer R W
Environmental Carcinogenesis Division, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711, USA.
Mutat Res. 1996 May;316(5-6):261-6. doi: 10.1016/s0921-8734(96)90008-9.
Spermatid micronuclei (MN) from Armenian hamsters in different age groups were compared with regard to frequencies and kinetochore status (presence or absence) as determined with immunofluorescent staining. Six thousand cells analyzed from each of fifteen young animals (3 months) revealed a group mean frequency of 0.45 MN/1000 spermatids; kinetochore staining was uniformly negative. Six thousand cells scored from each of fifteen older animals (2 years) revealed a group mean frequency of 1.00 MN/1000 spermatids. Most of the MN in these animals were negative for kinetochore staining, although a significant representation of MN with positive kinetochore staining was also observed. The results indicate that frequencies of spermatid MN increase with advancing age, and suggest that the increase is due to significant elevations in both chromosome breakage and chromosome loss.
通过免疫荧光染色，对不同年龄组亚美尼亚仓鼠的精子细胞微核（MN）频率和动粒状态（存在或不存在）进行了比较。对15只年轻动物（3个月）中的每只动物分析6000个细胞，结果显示每组平均频率为0.45个微核/1000个精子细胞；动粒染色均为阴性。对15只老年动物（2岁）中的每只动物计数6000个细胞，结果显示每组平均频率为1.00个微核/1000个精子细胞。这些动物中的大多数微核动粒染色为阴性，不过也观察到相当一部分微核动粒染色呈阳性。结果表明，精子细胞微核频率随年龄增长而增加，并且提示这种增加是由于染色体断裂和染色体丢失均显著增加所致。
