Vinayak R, Anderson P, McCollum C, Hampel A
Applied Biosystems Inc., Foster City, CA 94404.
Nucleic Acids Res. 1992 Mar 25;20(6):1265-9. doi: 10.1093/nar/20.6.1265.
The exocyclic amine protecting groups in oligonucleotide synthesis which require 8-16 hours at 55 degrees C for deprotection in ammonia have been replaced with more labile base protecting groups (dimethylformamidine for adenine and guanine and isobutyryl for cytosine). Using these fast oligonucleotide deprotecting groups which require 2-3 hours at 55 degrees C for complete deprotection, a new set of cyanoethyl phosphoramidite ribonucleoside monomers and supports has been developed. Ribozymes and substrate RNAs which were synthesized with these phosphoramidites were assayed and were found to have full catalytic (biological) activity.
在寡核苷酸合成中，那些在55摄氏度下于氨中脱保护需要8至16小时的环外胺保护基团，已被更易水解的碱基保护基团（腺嘌呤和鸟嘌呤用二甲基甲脒，胞嘧啶用异丁酰基）所取代。使用这些在55摄氏度下仅需2至3小时即可完全脱保护的快速寡核苷酸脱保护基团，已开发出一组新的氰乙基亚磷酰胺核糖核苷单体及载体。用这些亚磷酰胺合成的核酶和底物RNA经检测发现具有完全的催化（生物）活性。
