通过高效毛细管凝胶电泳快速分离和纯化寡核苷酸。
Rapid separation and purification of oligonucleotides by high-performance capillary gel electrophoresis.
作者信息
Cohen A S, Najarian D R, Paulus A, Guttman A, Smith J A, Karger B L
机构信息
Barnett Institute, Northeastern University, Boston, MA 02115.
出版信息
Proc Natl Acad Sci U S A. 1988 Dec;85(24):9660-3. doi: 10.1073/pnas.85.24.9660.
Picomole amounts of oligodeoxynucleotides [polydeoxyadenylic acids, (dA)40-60] were baseline resolved and analyzed in less than 8 min by high-performance capillary electrophoresis with polyacrylamide gels. In addition, fast analysis of a crude 70-mer oligodeoxynucleotide and a slab gel-purified 99-mer oligodeoxynucleotide was accomplished, demonstrating the ability of high-performance capillary electrophoresis to characterize rapidly synthesized oligonucleotides. Besides analytical separations, 800 ng of a primer (20-mer) was isolated in less than 20 min. The purified species was collected in water and subsequently used as a probe in a standard dot-blot analysis. The use of high-performance capillary electrophoresis for the analysis and purification of a variety of biopolymers is simple, rapid, and has the potential for automation.
皮摩尔量的寡聚脱氧核苷酸[聚脱氧腺苷酸，(dA)40 - 60]通过聚丙烯酰胺凝胶高效毛细管电泳在不到8分钟内实现基线分离和分析。此外，还完成了对粗制70聚体寡聚脱氧核苷酸和平板凝胶纯化的99聚体寡聚脱氧核苷酸的快速分析，证明了高效毛细管电泳对快速合成的寡核苷酸进行表征的能力。除了分析分离外，在不到20分钟内分离出了800纳克引物(20聚体)。纯化后的物质收集在水中，随后用作标准斑点印迹分析中的探针。使用高效毛细管电泳对各种生物聚合物进行分析和纯化简单、快速，并且具有自动化的潜力。
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