Kibbelaar R E, Mulder J W, van Kamp H, Dreef E J, Wessels H W, Beverstock G C, Haak H L, Raap A K, Kluin P M
Department of Pathology, University of Leiden, The Netherlands.
Genes Chromosomes Cancer. 1992 Mar;4(2):128-34. doi: 10.1002/gcc.2870040205.
Bone marrow cells of four patients with t(1;7) and myelodysplasia or acute myeloid leukemia were analyzed using nonradioactive in situ hydridisation. As probes, centromeric alphoid DNA sequences of chromosomes 1 and 7, a satellite DNA probe for 1q12, and chromosome-specific libraries of chromosomes 1 and 7 were used. The breakpoints of the t(1;7)(p11;p11) as determined by banding analysis could be studied more accurately, and the recently proposed designation t(1;7)(cen;cen) was confirmed in all four cases. Colocalization of alphoid DNA sequences of chromosomes 1 and 7 by double target in situ hybridisation was demonstrated in metaphase cells and also in interphase nuclei. The in situ hybridisation method described is applicable for the screening of peripheral blood cells or archival material.
使用非放射性原位杂交技术对4例患有t(1;7)且伴有骨髓发育异常或急性髓细胞白血病的患者的骨髓细胞进行了分析。作为探针，使用了1号和7号染色体的着丝粒α卫星DNA序列、一个用于1q12的卫星DNA探针以及1号和7号染色体的染色体特异性文库。通过显带分析确定的t(1;7)(p11;p11)的断点能够得到更精确的研究，并且最近提出的命名t(1;7)(cen;cen)在所有4例病例中均得到证实。在中期细胞以及间期核中均证实了通过双靶标原位杂交技术使1号和7号染色体的α卫星DNA序列共定位。所描述的原位杂交方法适用于外周血细胞或存档材料的筛查。
