Rubin C M, Nesbit M E, Kim T H, Kersey J H, Arthur D C
Department of Pediatrics, University of Chicago, Illinois 60637.
Genes Chromosomes Cancer. 1992 Mar;4(2):141-5. doi: 10.1002/gcc.2870040207.
Patients undergoing bone marrow transplantation often receive total body or total lymphoid irradiation as part of the conditioning regimen prior to marrow infusion. The cytogenetic effects of this therapy on skin fibroblasts were studied. Fibroblast cultures from eight skin biopsies were harvested in early passages for G-banded chromosome analysis. Four biopsies were from three patients who had high-dose cyclophosphamide and total body radiotherapy; one was from within and one was from outside the radiation field of a patient who had high-dose cyclophosphamide and lymphoid radiotherapy, one was from a patient who had combination chemotherapy alone, and one was from a normal control. No abnormal mitoses were found in the control or the patient who had chemotherapy alone, and only two of 30 mitoses from skin outside the lymphoid radiotherapy field were abnormal. However, most cells (49-88%) from five biopsies within radiotherapy fields were abnormal. Typically, abnormal karyotypes were pseudodiploid and contained multiple balanced rearrangements, of which reciprocal translocations were most common. The data indicate that the radiotherapy used for bone marrow transplantation induces extensive, sustained chromosome abnormalities in vivo in skin fibroblasts.
接受骨髓移植的患者在骨髓输注前，通常会接受全身或全淋巴照射，作为预处理方案的一部分。研究了这种疗法对皮肤成纤维细胞的细胞遗传学影响。从8份皮肤活检样本的早期传代培养物中收获成纤维细胞，用于G带染色体分析。4份活检样本取自3名接受大剂量环磷酰胺和全身放疗的患者；1份取自1名接受大剂量环磷酰胺和淋巴放疗患者的放疗区域内，1份取自放疗区域外，1份取自仅接受联合化疗的患者，1份取自正常对照。在对照组或仅接受化疗的患者中未发现异常有丝分裂，在淋巴放疗区域外的皮肤样本中，30个有丝分裂中仅有2个异常。然而，放疗区域内5份活检样本中的大多数细胞（49%-88%）异常。典型的异常核型为假二倍体，包含多个平衡重排，其中相互易位最为常见。数据表明，用于骨髓移植的放疗可在体内诱导皮肤成纤维细胞出现广泛、持续的染色体异常。
