Shariff A, Luna E J
Cell Biology Group, Worcester Foundation for Experimental Biology, Shresbury, MA 01545.
Science. 1992 Apr 10;256(5054):245-7. doi: 10.1126/science.1373523.
Diacylglycerols, which are generated during phospholipase-catalyzed hydrolysis of phospholipids, stimulated actin polymerization in the presence of highly purified plasma membranes from the cellular slime mold Dictyostelium discoideum. The increased rate of actin polymerization apparently resulted from de novo formation of actin nucleation sites rather than uncapping of existing filament ends, because the membranes lacked detectable endogenous actin. The increased actin nucleation was mediated by a peripheral membrane component other than protein kinase C, the classical target of diacylglycerol action. These results indicate that diacylglycerols increase actin nucleation at plasma membranes and suggest a mechanism whereby signal transduction pathways may control cytoskeletal assembly.
二酰基甘油是在磷脂酶催化磷脂水解过程中产生的，在存在来自细胞黏菌盘基网柄菌的高度纯化质膜的情况下，它能刺激肌动蛋白聚合。肌动蛋白聚合速率的增加显然是由于肌动蛋白成核位点的从头形成，而不是现有丝端的去封闭，因为这些膜缺乏可检测到的内源性肌动蛋白。肌动蛋白成核增加是由蛋白激酶C以外的一种外周膜成分介导的，蛋白激酶C是二酰基甘油作用的经典靶点。这些结果表明二酰基甘油增加质膜处的肌动蛋白成核，并提示了一种信号转导途径可能控制细胞骨架组装的机制。
