Ploeg R J, Boudjema K, Marsh D, Bruijn J A, Gooszen H G, Southard J H, Belzer F O
Department of Surgery, University of Wisconsin, Madison 53792.
Transplantation. 1992 Apr;53(4):735-41. doi: 10.1097/00007890-199204000-00005.
The role of hydroxyethyl starch (HES), the colloid component of the UW solution, was tested in canine pancreas preservation. Segmental pancreatic autografts were preserved for 48 hr cold storage with UW solution with HES (group 1) or UW solution without HES (group 2). After preservation, the pancreas was transplanted, and survival, serum glucose, serum amylase, intravenous glucose tolerance tests, tissue water content, and histology were compared between groups. In group 1 (with HES), 9/10 dogs were long-term survivors with one dog dying due to causes unrelated to preservation failure. In group 2 (without HES), 3/6 dogs died due to graft loss within one week posttransplant (P = 0.01). No graft failure occurred in group 1 (0/9) versus graft loss in 4/6 dogs in group 2 (P = 0.04). All animals in group 1 (with HES) showed normal serum glucose and amylase concentrations postoperatively, normal tissue water values after preservation, k values comparable to those observed after segmental autotransplantation without preservation, and relatively good histology. In group 2 (without HES), in 4/6 dogs graft failure occurred that led to the death (3 dogs) of the animals or to a diabetic state (1 dog). After 48-hr cold storage without HES, a significant increase in tissue water content, glucose and amylase levels was seen. After transplantation, hyperglycemia, hyperamylasemia, and clinical diabetes were observed in 4/6 dogs. Autopsy and histological evaluation showed evidence of thrombosis and ischemic insult. Two of 6 dogs in group 2 remained normoglycemic during follow-up with borderline k values. The results suggested that for consistently successful 48-hr preservation of the pancreas, HES is an important component of the UW solution. Although a colloid may not be essential for short-term preservation of kidney and liver, it appears to be an important factor in successful pancreas preservation.
UW 溶液中的胶体成分羟乙基淀粉（HES）在犬胰腺保存中的作用进行了测试。将节段性胰腺自体移植用含 HES 的 UW 溶液（第 1 组）或不含 HES 的 UW 溶液（第 2 组）冷藏保存 48 小时。保存后，进行胰腺移植，并比较两组之间的存活率、血清葡萄糖、血清淀粉酶、静脉葡萄糖耐量试验、组织含水量和组织学情况。在第 1 组（含 HES）中，10 只狗中有 9 只长期存活，1 只狗因与保存失败无关的原因死亡。在第 2 组（不含 HES）中，6 只狗中有 3 只在移植后一周内死于移植物丢失（P = 0.01）。第 1 组未发生移植物失败（0/9），而第 2 组 6 只狗中有 4 只发生移植物丢失（P = 0.04）。第 1 组（含 HES）的所有动物术后血清葡萄糖和淀粉酶浓度正常，保存后组织含水量值正常，k 值与未保存的节段性自体移植后观察到的值相当，组织学情况相对良好。在第 2 组（不含 HES）中，6 只狗中有 4 只发生移植物失败，导致动物死亡（3 只）或出现糖尿病状态（1 只）。在无 HES 的情况下冷藏 48 小时后，组织含水量、葡萄糖和淀粉酶水平显著升高。移植后，6 只狗中有 4 只出现高血糖、高淀粉酶血症和临床糖尿病。尸检和组织学评估显示有血栓形成和缺血性损伤的证据。第 2 组 6 只狗中有 2 只在随访期间血糖正常，k 值临界。结果表明，为了胰腺持续成功保存 48 小时，HES 是 UW 溶液的重要成分。虽然胶体对于肾脏和肝脏的短期保存可能不是必需的，但它似乎是胰腺成功保存的一个重要因素。
