Ar'Rajab A, Ahrén B, Sundberg R, Bengmark S
Department of Surgery, Lund University, Sweden.
Transplantation. 1991 Jul;52(1):34-8. doi: 10.1097/00007890-199107000-00007.
The value of colloid in preservation of the liver by cold storage has not yet been fully clarified. Therefore, we studied the effects of colloid on cell swelling, liver weight, and bile production after cold storage in rat liver tissue slices and isolated rabbit liver. In rat liver tissue slices cold-stored for 24 hr in UW solution, total tissue water (TTW) was the same as in the control freshly unpreserved tissue and omitting the colloid (hydroxyethyl starch) from the UW solution did not affect the TTW. However, after cold storage for 24 hr in Perfadex, TTW was markedly increased (by 100%, P less than 0.001). Omitting the colloid in this solution, dextran, or replacing it with hydroxyethyl starch, did not affect this increase in TTW. Thus, the hypothermia-induced cell swelling evident after preservation in Perfadex was not prevented by colloid. Rabbit liver cold-stored in UW solution for 24 hr lost 15.4 +/- 4.7% of weight, but omitting the colloid from UW solution decreased this weight loss to 3.1 +/- 3% (P less than 0.01). In contrast, rabbit livers cold-preserved in colloid-free Perfadex gained 23.3 +/- 5.7% in weight. Adding colloid, either dextran or hydroxyethyl starch, decreased significantly this weight gain, to 9 +/- 3.7% and 10.4 +/- 1.8%, respectively (P less than 0.01), probably as a result of colloid osmotic pressure, preventing the interstitial edema. Rabbit livers preserved for 24 hr in UW solution, with or without colloid, produced the same amount of bile as control unpreserved livers. In contrast, livers preserved in colloid-free Perfadex for 24 hr had a markedly impaired bile production (3.9 +/- 0.9 ml/100 g) as compared with control livers (15.5 +/- 2.6 ml/100 g, P less than 0.01). Colloid partially restored this impaired bile production, to 8 +/- 1.4 mg/100 g by dextran and to 8.5 +/- 1.7 ml/100 g by hydroxyethyl starch, respectively (P less than 0.01). Thus, although colloids do not prevent the hypothermia-induced cell swelling, they prevent the development of interstitial edema, and, hence, improve the liver function.
胶体在肝脏冷藏保存中的作用尚未完全明确。因此，我们研究了胶体对大鼠肝组织切片和离体兔肝冷藏后细胞肿胀、肝脏重量及胆汁生成的影响。在UW溶液中冷藏24小时的大鼠肝组织切片，其总组织水（TTW）与未冷藏的新鲜对照组织相同，且从UW溶液中省略胶体（羟乙基淀粉）对TTW无影响。然而，在Perfadex中冷藏24小时后，TTW显著增加（增加100%，P<0.001）。在该溶液中省略胶体右旋糖酐或用羟乙基淀粉替代它，均不影响TTW的增加。因此，在Perfadex中保存后出现的低温诱导细胞肿胀并未因胶体而得到预防。在UW溶液中冷藏24小时的兔肝重量减轻了15.4±4.7%，但从UW溶液中省略胶体可使这种重量减轻降至3.1±3%（P<0.01）。相反，在无胶体的Perfadex中冷藏的兔肝重量增加了23.3±5.7%。添加胶体，无论是右旋糖酐还是羟乙基淀粉，均可使这种重量增加显著降低，分别降至9±3.7%和10.4±1.8%（P<0.01），这可能是由于胶体渗透压防止了间质水肿。在UW溶液中保存24小时的兔肝，无论有无胶体，其胆汁生成量与未保存的对照肝相同。相反，在无胶体的Perfadex中保存24小时的肝脏胆汁生成明显受损（3.9±0.9 ml/100 g），与对照肝脏（15.5±2.6 ml/100 g，P<0.01）相比。胶体部分恢复了这种受损的胆汁生成，右旋糖酐使其恢复至8±1.4 mg/100 g，羟乙基淀粉使其恢复至8.5±1.7 ml/100 g，分别（P<0.01）。因此，尽管胶体不能预防低温诱导的细胞肿胀，但它们可防止间质水肿的发展，从而改善肝功能。
