Ansai S I, Katagata Y, Yoshikawa K I, Hozumi Y, Aso K
Department of Dermatology, Faculty of Medicine, Yamagata University, Japan.
Arch Dermatol Res. 1993;285(1-2):6-12. doi: 10.1007/BF00370816.
Keratin specificity analyses of eight anti-keratin antibodies (34 beta B4 (K1), 35 beta H11 (K8), Ks 13.1 (K13), Ks 19.1 (K19), PKK1, LP34 (CK1), KL1 and AE1) using keratin protein derived from normal thigh epidermis, normal parotid gland and a human squamous cell carcinoma cell line (HSC-5) were performed, and compared with those described in the data sheets. The reactivities of LP34, KL1 and PKK1 were markedly different from those mentioned in the data sheets. The immunostaining pattern of these antibodies in normal skin using formalin-fixed and paraffin-embedded tissue specimens was also examined. The staining patterns of suprabasal keratinocytes (K1, K13, CK1 and KL1 positive), basal cells of the epidermis (PKK1 and AE1 positive), inner cells of the ducts (K8, K13, CK1, KL1 and AE1 positive) and secretory cells of sweat glands (K8, K19, PKK1, KL1 and AE1 positive), mature cells (K8 and KL1 positive) and peripheral cells (CK1, KL1 and AE1 positive) of sebaceous glands and outer root sheaths (PKK1, CK1, KL1 and AE1 positive) were specific. Thus, we conclude that the differentiation of epidermis and skin appendages is possible by immunostaining with these eight anti-keratin antibodies using formalin-fixed and paraffin-embedded tissue specimens with proper protease pretreatment.
使用源自正常大腿表皮、正常腮腺和人鳞状细胞癌细胞系（HSC - 5）的角蛋白，对8种抗角蛋白抗体（34βB4（K1）、35βH11（K8）、Ks 13.1（K13）、Ks 19.1（K19）、PKK1、LP34（CK1）、KL1和AE1）进行了角蛋白特异性分析，并与数据表中描述的结果进行了比较。LP34、KL1和PKK1的反应性与数据表中提到的明显不同。还使用福尔马林固定和石蜡包埋的组织标本检查了这些抗体在正常皮肤中的免疫染色模式。基底上层角质形成细胞（K1、K13、CK1和KL1阳性）、表皮基底细胞（PKK1和AE1阳性）、导管内层细胞（K8、K13、CK1、KL1和AE1阳性）、汗腺分泌细胞（K8、K19、PKK1、KL1和AE1阳性）、皮脂腺成熟细胞（K8和KL1阳性）和外周细胞（CK1、KL1和AE1阳性）以及外根鞘（PKK1、CK1、KL1和AE1阳性）的染色模式具有特异性。因此，我们得出结论，通过使用适当蛋白酶预处理的福尔马林固定和石蜡包埋组织标本，用这8种抗角蛋白抗体进行免疫染色，可以实现表皮和皮肤附属器的分化。
