Rao J P, Sodja A
Department of Biological Sciences, Wayne State University, Detroit, Michigan 48208.
Biochem Biophys Res Commun. 1992 Apr 15;184(1):400-7. doi: 10.1016/0006-291x(92)91207-7.
Previously we uncovered a 0.45-kb transcript within the 3' end transcribed untranslated region (3'UTR) of actin gene at 5C3-4 (act5C) of Drosophila melanogaster. We report here that its sequence bears no similarity to the known DNA or protein sequences. This and act5C transcripts are loaded on different polyribosomal classes. Gel retardation experiments performed with this fragment and several others from act5C reveal no DNA binding activity. The 0.45-kb transcript, initially isolated from different developmental stages of D. melanogaster embryogenesis, is also expressed in Drosophila Kc tissue culture cells, which will be used in transformation experiments designed to identify regulatory features of the nested gene and its possible interaction at some level with its "host" act5C gene.
此前，我们在黑腹果蝇5C3 - 4（act5C）肌动蛋白基因的3'端转录非翻译区（3'UTR）内发现了一个0.45 kb的转录本。我们在此报告，其序列与已知的DNA或蛋白质序列没有相似性。该转录本和act5C转录本加载在不同的多核糖体类别上。用该片段和act5C的其他几个片段进行的凝胶阻滞实验未显示DNA结合活性。最初从黑腹果蝇胚胎发育的不同阶段分离出的0.45 kb转录本，在果蝇Kc组织培养细胞中也有表达，这些细胞将用于转化实验，以确定嵌套基因的调控特征及其在某些水平上与其“宿主”act5C基因的可能相互作用。
