Hasan G
Molecular Biology Unit, Tata Institute of Fundamental Research, Bombay, India.
Proc Natl Acad Sci U S A. 1990 Nov;87(22):9037-41. doi: 10.1073/pnas.87.22.9037.
An olfactory gene olfE, which affects response to benzaldehyde in larvae and adults of Drosophila melanogaster, has been mapped between two breakpoints on the X chromosome. The breakpoints have been shown to lie at a distance no greater than 25 kilobases (kb). A 14-kb genomic fragment from this region has been used for germ-line transformation of olfE mutant flies, and in one of three transformant lines obtained, rescue of the olfE phenotype is observed by two separate behavioral assays. Transcript analysis of the region that rescues the olfE phenotype has shown one major transcript at 5.4 kb and a minor one at 1.7 kb. Both of these transcripts are probably alternatively spliced products of the olfE gene. A developmental and tissue-specific profile of the 5.4-kb olfE message has shown that it is present at all developmental stages, suggesting that the gene may be multifunctional.
一种嗅觉基因olfE，它影响黑腹果蝇幼虫和成虫对苯甲醛的反应，已被定位在X染色体上的两个断点之间。这些断点已被证明位于不超过25千碱基（kb）的距离处。来自该区域的一个14 kb基因组片段已用于olfE突变果蝇的种系转化，并且在获得的三个转化系之一中，通过两种独立的行为测定观察到olfE表型的拯救。对拯救olfE表型的区域进行转录本分析，发现一个主要转录本为5.4 kb，一个次要转录本为1.7 kb。这两个转录本可能都是olfE基因的可变剪接产物。5.4 kb olfE信使RNA的发育和组织特异性图谱表明，它在所有发育阶段都存在，这表明该基因可能具有多种功能。
