Jung R, Engelhart G, Herbolt B, Jäckh R, Müller W
Department of Toxicology, Hoechst AG, Frankfurt, Germany.
Mutat Res. 1992 Apr;278(4):265-70. doi: 10.1016/s0165-1218(10)80006-0.
Thirty compounds of various chemical classes were investigated for mutagenicity in a collaborative study (3 laboratories) using Salmonella typhimurium TA102. With 5 compounds, namely hydrazine sulfate, phenylhydrazine, hydralazine, glutardialdehyde and glyoxal, mutagenicity was detected by all laboratories. Formaldehyde was assessed as weakly mutagenic in only 1 of 3 laboratories. The remaining 24 agents were uniformly described as non-genotoxic in TA102. In spite of the overall good qualitative agreement in the mutagenicity results between the 3 laboratories some quantitative discrepancies occurred in the dose response of the mutagenic compounds. Varying inter- and intra-laboratory differences in the spontaneous rate of revertants were obtained. The usefulness of the tester strain TA102 in routine mutagenicity testing is discussed.
在一项合作研究（3个实验室）中，使用鼠伤寒沙门氏菌TA102对30种不同化学类别的化合物进行了致突变性研究。对于5种化合物，即硫酸肼、苯肼、肼屈嗪、戊二醛和乙二醛，所有实验室均检测到了致突变性。甲醛仅在3个实验室中的1个被评估为弱致突变性。其余24种试剂在TA102中均被一致描述为非遗传毒性。尽管3个实验室之间致突变性结果在总体定性上吻合良好，但在致突变化合物的剂量反应方面仍存在一些定量差异。各实验室间和实验室内部的回复突变体自发率存在差异。文中讨论了测试菌株TA102在常规致突变性测试中的实用性。
