Vernet M, Bonnerot C, Briand P, Nicolas J F
Laboratoire de Génétique et Pathologie Expérimentales, INSERM Institut Cochin de Génétique Moléculaire, Paris, France.
Mech Dev. 1992 Feb;36(3):129-39. doi: 10.1016/0925-4773(92)90064-q.
LacZ DNA and LacZ RNA were microinjected during the first cleavages of embryos. LacZ DNA was not expressed before 18-19 h post insemination (hpi) but LacZ RNA was translated. Before 22 hpi LacZ DNA was expressed in the pronuclei of the one-cell embryos and the polypeptides of the minor, but not the major activation period of the genome were synthesized. This suggests a negative control of transcription before 18-19 hpi and demonstrates that its resumption is independent of the first cleavage and of the major activation of the genome. At the time of the minor activation the eggs contain the trans-acting elements to express a variety of genes that they do not express. It may indicate that, the minor and the major activation of the genome are differently controlled.
在胚胎的第一次卵裂期间显微注射了LacZ DNA和LacZ RNA。LacZ DNA在授精后18 - 19小时（hpi）之前未表达，但LacZ RNA被翻译。在22 hpi之前，LacZ DNA在单细胞胚胎的原核中表达，并且合成了基因组次要激活期而非主要激活期的多肽。这表明在18 - 19 hpi之前存在转录的负调控，并证明其恢复独立于第一次卵裂和基因组的主要激活。在次要激活时，卵中含有可表达多种它们未表达基因的反式作用元件。这可能表明，基因组的次要激活和主要激活受到不同的调控。
