cis-acting elements that mediate the negative regulation of Moloney murine leukemia virus in mouse early embryos.
作者信息
Vernet M, Cebrian J
机构信息
Institut Cochin de Génétique Molécularire, Laboratoire de Génétique et Pathologie Expérimentales, Unité 380 de l'Institut National de la Santé et de la Recherche Médicale, Paris, France.
出版信息
J Virol. 1996 Aug;70(8):5630-3. doi: 10.1128/JVI.70.8.5630-5633.1996.
Abstract
We have addressed the question of the nature of Moloney murine leukemia virus (MoMuLV) repression in mouse embryos by assaying for the transient expression of MoMuLV-derived constructs microinjected into early cleavage embryos. We show that the same cis-acting DNA sequences responsible for the block in MoMuLV expression in embryonal carcinoma cell lines operate in early embryos: (i) the MoMuLV long terminal repeat is nonfunctional, and (ii) the +147 to +163 repressor binding site, or negative regulatory element, negatively regulates the expression from an active promoter.
摘要
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cis-acting elements that mediate the negative regulation of Moloney murine leukemia virus in mouse early embryos.
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