• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
cis-acting elements that mediate the negative regulation of Moloney murine leukemia virus in mouse early embryos.在小鼠早期胚胎中介导莫洛尼鼠白血病病毒负调控的顺式作用元件。
J Virol. 1996 Aug;70(8):5630-3. doi: 10.1128/JVI.70.8.5630-5633.1996.
2
Two blocks in Moloney murine leukemia virus expression in undifferentiated F9 embryonal carcinoma cells as determined by transient expression assays.通过瞬时表达分析确定，莫洛尼鼠白血病病毒在未分化的F9胚胎癌细胞中的表达受两个阻断因素影响。
J Virol. 1989 May;63(5):2317-24. doi: 10.1128/JVI.63.5.2317-2324.1989.
3
A single mutation in one of the CORE elements of Moloney murine leukemia virus reduced binding of a 42-kDa T lymphoma cell nuclear factor but did not affect lymphomagenesis.
Mol Carcinog. 1990;3(2):93-102. doi: 10.1002/mc.2940030207.
4
Replication of enhancer-deficient amphotropic murine leukemia virus in human cells.增强子缺陷型双嗜性鼠白血病病毒在人细胞中的复制
Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10898-903. doi: 10.1073/pnas.191182098. Epub 2001 Sep 4.
5
Negative regulatory element associated with potentially functional promoter and enhancer elements in the long terminal repeats of endogenous murine leukemia virus-related proviral sequences.与内源性鼠白血病病毒相关前病毒序列长末端重复序列中潜在功能性启动子和增强子元件相关的负调控元件。
J Virol. 1989 Jun;63(6):2746-57. doi: 10.1128/JVI.63.6.2746-2757.1989.
6
Novel retroviral vectors for efficient expression of the multidrug resistance (mdr-1) gene in early hematopoietic cells.用于在早期造血细胞中高效表达多药耐药（mdr-1）基因的新型逆转录病毒载体。
J Virol. 1995 Dec;69(12):7541-7. doi: 10.1128/JVI.69.12.7541-7547.1995.
7
Substitution of murine transthyretin (prealbumin) regulatory sequences into the Moloney murine leukemia virus long terminal repeat yields infectious virus with altered biological properties.将小鼠甲状腺素运载蛋白（前白蛋白）调控序列替换莫洛尼氏鼠白血病病毒长末端重复序列，可产生具有改变生物学特性的传染性病毒。
J Virol. 1990 Dec;64(12):6130-40. doi: 10.1128/JVI.64.12.6130-6140.1990.
8
Analysis of the binding proteins and activity of the long terminal repeat of Moloney murine leukemia virus during differentiation of mouse embryonal carcinoma cells.莫洛尼鼠白血病病毒长末端重复序列在小鼠胚胎癌细胞分化过程中的结合蛋白及活性分析
J Virol. 1991 Jun;65(6):2979-86. doi: 10.1128/JVI.65.6.2979-2986.1991.
9
The Moloney murine leukemia virus enhancer and its flanking sequences collaborate to determine virulence in T-cell lymphomagenesis.
Mol Carcinog. 1991;4(1):72-80. doi: 10.1002/mc.2940040111.
10
Slipped DNA structures within the enhancer region of the Moloney murine leukemia virus.莫洛尼鼠白血病病毒增强子区域内的DNA滑动结构
Biochem Biophys Res Commun. 1988 Oct 14;156(1):417-23. doi: 10.1016/s0006-291x(88)80857-x.

引用本文的文献

1
Proviral silencing in embryonic stem cells requires the histone methyltransferase ESET.原病毒在胚胎干细胞中的沉默需要组蛋白甲基转移酶 ESET。
Nature. 2010 Apr 8;464(7290):927-31. doi: 10.1038/nature08858. Epub 2010 Feb 17.
2
Lack of shielding of primer binding site silencer-mediated repression of an internal promoter in a retrovirus vector by the putative insulators scs, BEAD-1, and HS4.假定的绝缘子scs、BEAD-1和HS4对逆转录病毒载体中内部启动子的引物结合位点沉默子介导的抑制作用缺乏屏蔽。
J Virol. 2000 Dec;74(24):11697-707. doi: 10.1128/jvi.74.24.11697-11707.2000.
3
Viral vectors for gene transfer: a review of their use in the treatment of human diseases.用于基因转移的病毒载体：其在人类疾病治疗中的应用综述
Drugs. 2000 Aug;60(2):249-71. doi: 10.2165/00003495-200060020-00002.

本文引用的文献

1
Characterization of the Moloney murine leukemia virus stem cell-specific repressor binding site.莫洛尼鼠白血病病毒干细胞特异性阻遏物结合位点的表征
Virology. 1993 Apr;193(2):690-9. doi: 10.1006/viro.1993.1177.
2
Application of LacZ gene fusions to preimplantation development.LacZ基因融合技术在植入前发育中的应用。
Methods Enzymol. 1993;225:434-51. doi: 10.1016/0076-6879(93)25030-6.
3
Stem cell factor binding to retrovirus primer binding site silencers.干细胞因子与逆转录病毒引物结合位点沉默子的结合。
J Virol. 1995 Feb;69(2):1142-9. doi: 10.1128/JVI.69.2.1142-1149.1995.
4
Non-function of a Moloney murine leukaemia virus regulatory sequence in F9 embryonal carcinoma cells.莫洛尼鼠白血病病毒调控序列在F9胚胎癌细胞中的无功能状态
Nature. 1984;308(5958):470-2. doi: 10.1038/308470a0.
5
Expression and regulation of Escherichia coli lacZ gene fusions in mammalian cells.大肠杆菌lacZ基因融合体在哺乳动物细胞中的表达与调控
J Mol Appl Genet. 1983;2(1):101-9.
6
De novo methylation and expression of retroviral genomes during mouse embryogenesis.小鼠胚胎发育过程中逆转录病毒基因组的从头甲基化与表达
Nature. 1982 Aug 12;298(5875):623-8. doi: 10.1038/298623a0.
7
A short amino acid sequence able to specify nuclear location.一段能够指定核定位的短氨基酸序列。
Cell. 1984 Dec;39(3 Pt 2):499-509. doi: 10.1016/0092-8674(84)90457-4.
8
Firefly luciferase gene: structure and expression in mammalian cells.萤火虫荧光素酶基因：在哺乳动物细胞中的结构与表达
Mol Cell Biol. 1987 Feb;7(2):725-37. doi: 10.1128/mcb.7.2.725-737.1987.
9
Chromosomal position or virus mutation permits retrovirus expression in embryonal carcinoma cells.染色体位置或病毒突变使得逆转录病毒能在胚胎癌细胞中表达。
Cell. 1986 Nov 7;47(3):391-9. doi: 10.1016/0092-8674(86)90596-9.
10
Proviral sequences that restrict retroviral expression in mouse embryonal carcinoma cells.在小鼠胚胎癌细胞中限制逆转录病毒表达的前病毒序列。
Mol Cell Biol. 1987 Oct;7(10):3775-84. doi: 10.1128/mcb.7.10.3775-3784.1987.

在小鼠早期胚胎中介导莫洛尼鼠白血病病毒负调控的顺式作用元件。

cis-acting elements that mediate the negative regulation of Moloney murine leukemia virus in mouse early embryos.

作者信息

Vernet M, Cebrian J

机构信息

Institut Cochin de Génétique Molécularire, Laboratoire de Génétique et Pathologie Expérimentales, Unité 380 de l'Institut National de la Santé et de la Recherche Médicale, Paris, France.

出版信息

J Virol. 1996 Aug;70(8):5630-3. doi: 10.1128/JVI.70.8.5630-5633.1996.

DOI:10.1128/JVI.70.8.5630-5633.1996
PMID:8764077
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC190523/
Abstract

We have addressed the question of the nature of Moloney murine leukemia virus (MoMuLV) repression in mouse embryos by assaying for the transient expression of MoMuLV-derived constructs microinjected into early cleavage embryos. We show that the same cis-acting DNA sequences responsible for the block in MoMuLV expression in embryonal carcinoma cell lines operate in early embryos: (i) the MoMuLV long terminal repeat is nonfunctional, and (ii) the +147 to +163 repressor binding site, or negative regulatory element, negatively regulates the expression from an active promoter.

摘要

我们通过检测显微注射到早期卵裂胚胎中的莫洛尼鼠白血病病毒（MoMuLV）衍生构建体的瞬时表达，探讨了小鼠胚胎中MoMuLV抑制的本质问题。我们发现，在胚胎癌细胞系中负责阻断MoMuLV表达的相同顺式作用DNA序列在早期胚胎中也起作用：（i）MoMuLV长末端重复序列无功能，以及（ii）+147至+163阻遏物结合位点或负调控元件对来自活性启动子的表达起负调控作用。