在小鼠早期胚胎中介导莫洛尼鼠白血病病毒负调控的顺式作用元件。
cis-acting elements that mediate the negative regulation of Moloney murine leukemia virus in mouse early embryos.
作者信息
Vernet M, Cebrian J
机构信息
Institut Cochin de Génétique Molécularire, Laboratoire de Génétique et Pathologie Expérimentales, Unité 380 de l'Institut National de la Santé et de la Recherche Médicale, Paris, France.
出版信息
J Virol. 1996 Aug;70(8):5630-3. doi: 10.1128/JVI.70.8.5630-5633.1996.
Abstract
We have addressed the question of the nature of Moloney murine leukemia virus (MoMuLV) repression in mouse embryos by assaying for the transient expression of MoMuLV-derived constructs microinjected into early cleavage embryos. We show that the same cis-acting DNA sequences responsible for the block in MoMuLV expression in embryonal carcinoma cell lines operate in early embryos: (i) the MoMuLV long terminal repeat is nonfunctional, and (ii) the +147 to +163 repressor binding site, or negative regulatory element, negatively regulates the expression from an active promoter.
摘要
我们通过检测显微注射到早期卵裂胚胎中的莫洛尼鼠白血病病毒（MoMuLV）衍生构建体的瞬时表达，探讨了小鼠胚胎中MoMuLV抑制的本质问题。我们发现，在胚胎癌细胞系中负责阻断MoMuLV表达的相同顺式作用DNA序列在早期胚胎中也起作用：（i）MoMuLV长末端重复序列无功能，以及（ii）+147至+163阻遏物结合位点或负调控元件对来自活性启动子的表达起负调控作用。
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本文引用的文献
1
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Virology. 1993 Apr;193(2):690-9. doi: 10.1006/viro.1993.1177.
2
Application of LacZ gene fusions to preimplantation development.LacZ基因融合技术在植入前发育中的应用。
Methods Enzymol. 1993;225:434-51. doi: 10.1016/0076-6879(93)25030-6.
3
Stem cell factor binding to retrovirus primer binding site silencers.干细胞因子与逆转录病毒引物结合位点沉默子的结合。
J Virol. 1995 Feb;69(2):1142-9. doi: 10.1128/JVI.69.2.1142-1149.1995.
4
Non-function of a Moloney murine leukaemia virus regulatory sequence in F9 embryonal carcinoma cells.莫洛尼鼠白血病病毒调控序列在F9胚胎癌细胞中的无功能状态
Nature. 1984;308(5958):470-2. doi: 10.1038/308470a0.
5
Expression and regulation of Escherichia coli lacZ gene fusions in mammalian cells.大肠杆菌lacZ基因融合体在哺乳动物细胞中的表达与调控
J Mol Appl Genet. 1983;2(1):101-9.
6
De novo methylation and expression of retroviral genomes during mouse embryogenesis.小鼠胚胎发育过程中逆转录病毒基因组的从头甲基化与表达
Nature. 1982 Aug 12;298(5875):623-8. doi: 10.1038/298623a0.
7
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Cell. 1984 Dec;39(3 Pt 2):499-509. doi: 10.1016/0092-8674(84)90457-4.
8
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9
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