Manova K, Paynton B V, Bachvarova R F
Department of Cell Biology and Anatomy, Cornell University Medical College, New York, NY 10021.
Mech Dev. 1992 Feb;36(3):141-52. doi: 10.1016/0925-4773(92)90065-r.
The expression of the mesoderm inducing factors, activins and TGF beta s, was characterized in 5 1/2-9 1/2 day mouse embryos and implantation sites by in situ hybridization. Activin beta A RNA was not detected within the embryo, but is expressed in nearby decidual cells from 5 to 7 days. Thus activin A could play a role within the embyro during gastrulation. Activin beta A is also expressed in more mesometrially located decidual cells from 6 to 9 1/2 days. Activin beta B and inhibin alpha RNAs were not detected, while a control tissue was highly positive. TGF beta 1 is expressed in the secondary decidual zone and in developing endothelial cells in the decidua and embryo. TGF beta 2 is expressed in the mesometrial decidua at 6 1/2 days and in the midline of the cranial neural plate.
通过原位杂交技术，对中胚层诱导因子激活素和转化生长因子β（TGFβ）在5.5至9.5天龄小鼠胚胎及着床部位的表达进行了特征分析。在胚胎内未检测到激活素βA RNA，但在5至7天龄时，其在附近的蜕膜细胞中表达。因此，激活素A可能在原肠胚形成过程中在胚胎内发挥作用。激活素βA在6至9.5天龄时，也在位于子宫系膜侧的蜕膜细胞中表达。未检测到激活素βB和抑制素α RNA，而对照组织呈强阳性。TGFβ1在次级蜕膜区以及蜕膜和胚胎中发育的内皮细胞中表达。TGFβ2在6.5天龄时在子宫系膜侧蜕膜以及颅神经板中线表达。
