Lu M, Tjerneld F, Johansson G, Albertsson P A
Department of Biochemistry, University of Lund, Sweden.
Bioseparation. 1991;2(4):247-55.
The graft modification of dextran with benzoyl groups has been studied. The factors that affect the degree of substitution of benzoyl dextran were investigated. Phase diagrams for aqueous two-phase systems composed of polyethylene glycol/benzoyl dextran and dextran/benzoyl dextran have been determined. Phase separation was also obtained in aqueous solution of two benzoyl dextran polymers with different degrees of substitution. A four-phase system was obtained with a mixture of polyethylene glycol, dextran and two kinds of benzoyl dextrans. The partitioning of methylene blue and a Procion yellow HE-3G dextran derivative were studied in polyethylene glycol/benzoyl dextran and dextran/benzoyl dextran two-phase systems and in systems of two benzoyl dextrans differing in degree of substitution. The proteins bovine serum albumin and glucose-6-phosphate dehydrogenase were partitioned in polyethylene glycol/benzoyl dextran aqueous two-phase systems and the effect of the degree of substitution of benzoyl dextran was studied. Chlorella pyrenoidosa, thylakoid membrane vesicles, plasma membrane vesicles and chloroplasts were partitioned in polyethylene glycol/benzoyl dextran and dextran/benzoyl dextran two-phase systems, and in a polyethylene glycol/dextran/benzoyl dextran four-phase system.
已对用苯甲酰基修饰葡聚糖进行了研究。考察了影响苯甲酰基葡聚糖取代度的因素。测定了由聚乙二醇/苯甲酰基葡聚糖和葡聚糖/苯甲酰基葡聚糖组成的双水相体系的相图。在具有不同取代度的两种苯甲酰基葡聚糖聚合物的水溶液中也获得了相分离。用聚乙二醇、葡聚糖和两种苯甲酰基葡聚糖的混合物得到了一个四相体系。研究了亚甲基蓝和一种普施安黄HE - 3G葡聚糖衍生物在聚乙二醇/苯甲酰基葡聚糖和葡聚糖/苯甲酰基葡聚糖双水相体系以及在取代度不同的两种苯甲酰基葡聚糖体系中的分配情况。研究了牛血清白蛋白和葡萄糖 - 6 - 磷酸脱氢酶在聚乙二醇/苯甲酰基葡聚糖双水相体系中的分配情况以及苯甲酰基葡聚糖取代度的影响。研究了小球藻、类囊体膜囊泡、质膜囊泡和叶绿体在聚乙二醇/苯甲酰基葡聚糖和葡聚糖/苯甲酰基葡聚糖双水相体系以及在聚乙二醇/葡聚糖/苯甲酰基葡聚糖四相体系中的分配情况。
