Crawley D E, McCormack D G, Evans T W
Department of Thoracic Medicine, National Heart and Lung Institute, London, UK.
Crit Care Med. 1992 May;20(5):641-4. doi: 10.1097/00003246-199205000-00016.
To investigate the effects of the intratracheal and iv administration of bleomycin on the contraction and endothelially dependent vasodilation of rat pulmonary arteries in vitro.
Prospective pharmacologic study.
National Heart and Lung Institute, London, UK.
Intratracheal saline, intratracheal and iv bleomycin.
Rats treated with intratracheal bleomycin developed a significant increase in mean lung wet/dry weight ratio (5.6 +/- 0.4 [SEM] vs. 3.9 +/- 0.1, p less than .05) when compared with saline-treated control animals, confirming the development of pulmonary edema. However, these rats displayed normal relaxant responses to the endothelially dependent vasodilator acetylcholine and a normal contractile response to phenylephrine in vitro. Intravenous bleomycin had no effect on either wet/dry weight ratio or the response to either drug.
Despite evidence for the loss of endothelial integrity that characterizes lung injury after intratracheal bleomycin, isolated pulmonary artery rings in vitro showed no loss of endothelial cell function. The role of the endothelium in modulating pulmonary ventilation/perfusion matching after lung injury is unclear.
研究气管内和静脉注射博来霉素对大鼠离体肺动脉收缩及内皮依赖性血管舒张的影响。
前瞻性药理学研究。
英国伦敦国家心肺研究所。
气管内注射生理盐水、气管内和静脉注射博来霉素。
与生理盐水处理的对照动物相比，气管内注射博来霉素的大鼠平均肺湿/干重比显著增加（5.6±0.4[标准误]对3.9±0.1，p<0.05），证实发生了肺水肿。然而，这些大鼠离体时对内皮依赖性血管舒张剂乙酰胆碱表现出正常的舒张反应，对去氧肾上腺素表现出正常的收缩反应。静脉注射博来霉素对湿/干重比或对任何一种药物的反应均无影响。
尽管有证据表明气管内注射博来霉素后肺损伤的特征是内皮完整性丧失，但离体肺动脉环显示内皮细胞功能并未丧失。肺损伤后内皮在调节肺通气/血流匹配中的作用尚不清楚。
