Halsell C B
Center for Neurological Sciences, University of Connecticut Health Center, Farmington 06030.
J Comp Neurol. 1992 Mar 1;317(1):57-78. doi: 10.1002/cne.903170105.
While gustation in the hamster has been extensively studied at the behavioral and physiological level, very little is known about the central anatomy of the taste system. The purpose of this study was to trace the connections of the parabrachial nucleus (PBN) in the golden Syrian hamster (Mesocricetus auratus) using wheat germ agglutinin-conjugated horseradish peroxidase. The PBN is the site of the second central synapse for the ascending gustatory system and receives taste afferents from the nucleus of the solitary tract. Following large injections into the PBN, anterogradely transported label was seen in the lateral hypothalamus, dorsal thalamus, bed nucleus of the stria terminalis, and amygdala. The anatomy of the two primary targets, the ventral posteromedial thalamus and central nucleus of the amygdala, is described based on Nissl-stained material, and acetylcholinesterase and NADH dehydrogenase histochemistry. Injections into these two regions revealed different patterns of efferents within the PBN. Following injections into the thalamus, retrogradely labelled cell bodies were distributed throughout the PBN subdivisions bilaterally, but concentrated in the central medial (CM) and external lateral (EL) subdivisions. Following injections into the amygdala, retrogradely labelled cell bodies were primarily in the ipsilateral PBN EL, while anterogradely transported label was distributed throughout much of the ipsilateral PBN. The majority of CM efferents projecting to the thalamus were elongate cells, whereas the majority of CM efferents to the amygdala were round-oval cells. These results indicate that the ascending central gustatory system changes from a serial pathway (nucleus of the solitary tract-PBN) to a parallel organization consisting of two major projections, the parabrachio-thalamo-cortical and parabrachio-amygdaloid pathways.
虽然已经在行为和生理水平上对仓鼠的味觉进行了广泛研究，但对于味觉系统的中枢解剖结构却知之甚少。本研究的目的是使用小麦胚凝集素结合辣根过氧化物酶来追踪金黄叙利亚仓鼠（Mesocricetus auratus）臂旁核（PBN）的连接。PBN是上行味觉系统第二个中枢突触的所在位置，并接收来自孤束核的味觉传入纤维。在向PBN进行大量注射后，在下丘脑外侧、背侧丘脑、终纹床核和杏仁核中可见顺行运输的标记物。基于尼氏染色材料、乙酰胆碱酯酶和NADH脱氢酶组织化学，描述了两个主要靶点——腹后内侧丘脑和杏仁核中央核的解剖结构。向这两个区域注射显示了PBN内不同的传出模式。向丘脑注射后，逆行标记的细胞体双侧分布于整个PBN亚区，但集中在中央内侧（CM）和外侧外侧（EL）亚区。向杏仁核注射后，逆行标记的细胞体主要位于同侧PBN的EL区，而顺行运输的标记物分布于同侧PBN的大部分区域。投射到丘脑的大多数CM传出纤维是细长细胞，而投射到杏仁核的大多数CM传出纤维是圆形 - 椭圆形细胞。这些结果表明，上行中枢味觉系统从串行通路（孤束核 - PBN）转变为由两条主要投射组成的平行组织，即臂旁 - 丘脑 - 皮质通路和臂旁 - 杏仁核通路。
