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使用人胰岛素样生长因子-I片段21-40及其互补肽对一种分子识别理论的批判性评估。

Critical evaluation of a theory of molecular recognition using human insulin-like-growth-factor-I fragment 21-40 and its complementary peptide.

作者信息

Beattie J, Flint D J

机构信息

Hannah Research Institute, Ayr, Scotland, U.K.

出版信息

Biochem J. 1992 Apr 15;283 ( Pt 2)(Pt 2):473-8. doi: 10.1042/bj2830473.

DOI:10.1042/bj2830473
PMID:1374232
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1131059/
Abstract

Using solid-phase methods we have synthesized human insulin-like-growth-factor-I (IGF-I) fragment 21-40 (IGF-I 21-40) and the peptide derived from the 5'----3' translation of the complementary nucleic acid of this peptide, 'I-FGI 20-40' (the complementary peptide). According to a recently proposed theory of molecular recognition, these two peptides should bind specifically to each other. We have tested this theory by using both solid- and solution-phase direct-binding assays for this complementary-peptide pair. We have also investigated the ability of I-FGI 20-40 to interfere with native IGF-I binding during radioimmunoassay (r.i.a.), radio-receptor (r.r.a.) assay and ligand-blot analysis of IGF-binding proteins. We have obtained no evidence of any interaction between IGF-I 21-40 and I-FGI 20-40 in either solid- or solution-phase assays. In addition, I-FGI 20-40 does not interfere in the assays used to detect IGF-I binding antibodies (r.i.a.), receptors (r.r.a.) or binding proteins (ligand blots). Our data therefore question the universality of this particular theory of molecular recognition.

摘要

我们采用固相方法合成了人胰岛素样生长因子-I（IGF-I）片段21 - 40（IGF-I 21 - 40）以及由该肽段互补核酸的5'----3'翻译衍生而来的肽段“I-FGI 20 - 40”（互补肽）。根据最近提出的分子识别理论，这两种肽段应该会特异性地相互结合。我们通过对这对互补肽段进行固相结合分析和溶液相结合分析来验证这一理论。我们还研究了I-FGI 20 - 40在放射免疫分析（r.i.a.）、放射受体分析（r.r.a.）以及IGF结合蛋白的配体印迹分析中干扰天然IGF-I结合的能力。在固相分析或溶液相分析中，我们均未获得IGF-I 21 - 40与I-FGI 20 - 40之间存在任何相互作用的证据。此外，I-FGI 20 - 40在用于检测IGF-I结合抗体（r.i.a.）、受体（r.r.a.）或结合蛋白（配体印迹）的分析中不会产生干扰。因此，我们的数据对这一特定分子识别理论的普遍性提出了质疑。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ea/1131059/0c73ad5227da/biochemj00137-0158-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ea/1131059/0c73ad5227da/biochemj00137-0158-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ea/1131059/0c73ad5227da/biochemj00137-0158-a.jpg

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