Berg E L, Magnani J, Warnock R A, Robinson M K, Butcher E C
Department of Pathology, Stanford University, CA 94305.
Biochem Biophys Res Commun. 1992 Apr 30;184(2):1048-55. doi: 10.1016/0006-291x(92)90697-j.
The L- and E-selectins are leukocyte and endothelial cell surface molecules which mediate leukocyte-endothelial cell adhesion by interacting with carbohydrate ligands. In the present study we find that L-selectin, like E-selectin, can interact with synthetic neoglycoproteins containing Sialyl Le(x) (Neu5Ac alpha 2-3Gal beta 1-4[Fuc alpha 1-3]GlcNAc beta-R), or Sialyl Le(a) (Neu5Ac-alpha 2-3Gal beta 1-3[Fuc alpha 1-4]GlcNAc beta-R). Additionally, both the E-selectin and L-selectin can bind the peripheral lymph node addressin, a high endothelial venule ligand for L-selectin. Despite overlapping interactions, the L- and E-selectins discriminate between their native ligands. The peripheral lymph node addressin is a preferential ligand for L-selectin; and furthermore, L-selectin expressing cells do not interact detectably with the cutaneous lymphocyte antigen, a native glycoprotein ligand for E-selectin found on a subset of lymphocytes associated with the skin.
L-选择素和E-选择素是白细胞和内皮细胞表面分子，它们通过与碳水化合物配体相互作用介导白细胞与内皮细胞的黏附。在本研究中，我们发现L-选择素与E-选择素一样，能够与含有唾液酸化路易斯寡糖（Neu5Acα2-3Galβ1-4[Fucα1-3]GlcNAcβ-R）或唾液酸化路易斯寡糖a（Neu5Ac-α2-3Galβ1-3[Fucα1-4]GlcNAcβ-R）的合成新糖蛋白相互作用。此外，E-选择素和L-选择素都能结合外周淋巴结地址素，这是L-选择素的一种高内皮微静脉配体。尽管存在重叠的相互作用，但L-选择素和E-选择素能区分它们的天然配体。外周淋巴结地址素是L-选择素的优先配体；此外，表达L-选择素的细胞与皮肤淋巴细胞抗原之间没有可检测到的相互作用，皮肤淋巴细胞抗原是在与皮肤相关的一部分淋巴细胞上发现的E-选择素的天然糖蛋白配体。
