Ercan M T, Kriegel H
Abteilung für Nuklearbiologie der Gesellschaft fäur Strahlen-und Unweltforschung mbH, München, Fed. Rep., Germany.
Int J Rad Appl Instrum B. 1992 Jan;19(1):101-2. doi: 10.1016/0883-2897(92)90191-z.
99mTc-dextran (D) was further evaluated in the present study as a lymphoscintigraphic agent compared to radiocolloids. It was injected intra-dermally into the web space between the second and third toes in both hind feet of two rabbits. 99mTc-nanocolloid (NC) was injected subcutaneously in both hind feet of two other rabbits. Popliteal lymph nodes were taken out and frozen in liquid nitrogen after the animals were sacrificed at 2 h post-injection. Three frozen sections in 10 microns thickness were prepared from each node for autoradiographic studies. The lymph node slices were exposed for 18 h using Ilford G 5 emulsion. The obtained autoradiographs showed that the distribution of 99mTc-D radioactivity within lymph nodes was more uniform indicating better tissue penetration compared to 99mTc-NC which remained mostly in the lymph canaliculi.
在本研究中，与放射性胶体相比，对99mTc-右旋糖酐（D）作为一种淋巴闪烁造影剂进行了进一步评估。将其皮内注射到两只兔子两只后足的第二和第三趾之间的蹼间隙中。将99mTc-纳米胶体（NC）皮下注射到另外两只兔子的两只后足中。在注射后2小时处死动物，取出腘窝淋巴结并在液氮中冷冻。从每个淋巴结制备三个10微米厚的冰冻切片用于放射自显影研究。使用伊尔福G 5乳剂将淋巴结切片曝光18小时。获得的放射自显影片显示，与主要留在淋巴管的99mTc-NC相比，99mTc-D放射性在淋巴结内的分布更均匀，表明组织穿透性更好。
