RYTER A
J Biophys Biochem Cytol. 1960 Oct;8(2):399-412.
Nuclear transformations induced in K12S and K12S(λ) by ultraviolet radiations and x-rays have been studied with ultrathin sections in the electron microscope. The nucleoplasm keeps its normal aspect during "fragmentation" and during "condensation" of the nucleus into the "vesicular" form. Serial sections show that the "fragmented" nucleus consists in reality of only one very tortuous vacuole. No difference either in the shape or in the fine structure of the nucleus could be observed between the lysogenic strain and the non-lysogenic strain. A high concentration of NaCl has a "condensation" effect on the fragmented nuclei and decreases the induction rate.
利用电子显微镜中的超薄切片研究了紫外线和X射线在K12S和K12S(λ)中诱导的核转变。在核“碎片化”以及核凝聚成“囊泡”形式的过程中,核质保持其正常形态。连续切片显示,“碎片化”的核实际上仅由一个非常曲折的液泡组成。在溶原性菌株和非溶原性菌株之间,未观察到核的形状或精细结构有任何差异。高浓度的NaCl对碎片化的核有“凝聚”作用,并降低诱导率。