Sakamoto S, Goto S, Ito H, Hirano A
Bluestone Laboratory, Montefiore Medical Center, Bronx, NY 10467.
Neurology. 1992 May;42(5):1071-5. doi: 10.1212/wnl.42.5.1071.
We designed this study to evaluate the expression of substance P (SP) in the striatum and the globus pallidus in normal controls, idiopathic Parkinson's disease (PD), and striatonigral degeneration (SND). We determined immunohistochemically the expression of SP in formalin-fixed, paraffin-embedded brain tissues using the free-floating method. In normal controls, we observed SP-like immunoreactivity in the internal segment of the globus pallidus, where the SP-positive fibers showed typical "woolly fiber" patterns. SP expression in the striatum had the characteristic patchwork of striosomes. Patients with PD showed the normal distribution pattern of SP. By comparison, in patients with SND, there was a striking diminution of SP in the putamen, while the SP-positive patches persisted in the caudate nucleus. This study represents the first unequivocal immunohistochemical demonstration of SP-positive striosomes in the human striatum.
我们开展这项研究，旨在评估正常对照、特发性帕金森病（PD）及纹状体黑质变性（SND）患者纹状体和苍白球中P物质（SP）的表达情况。我们采用漂片法，通过免疫组织化学方法测定福尔马林固定、石蜡包埋脑组织中SP的表达。在正常对照中，我们在苍白球内侧段观察到SP样免疫反应性，其中SP阳性纤维呈现典型的“毛状纤维”模式。纹状体中的SP表达具有纹状体小体的特征性镶嵌分布。PD患者的SP呈现正常分布模式。相比之下，SND患者的壳核中SP显著减少，而尾状核中的SP阳性斑块持续存在。本研究首次明确地通过免疫组织化学方法证实了人类纹状体中存在SP阳性纹状体小体。
