Besson M J, Graybiel A M, Quinn B
Institut des Neurosciences, CNRS, Université Pierre et Marie Curie, Paris, France.
Neuroscience. 1990;39(1):33-58. doi: 10.1016/0306-4522(90)90220-x.
The expression of tachykinin-like and opioid-like peptides was studied in medium-sized neurons of the caudate nucleus in tissue from adult cats pretreated with colchicine. Two methods, a serial thin-section peroxidase-antiperoxidase technique and a two-fluorochrome single-section technique, were applied. Quantitative estimates were made mainly with the peroxidase-antiperoxidase method. The numbers of neurons expressing substance P-like, dynorphin B-like, and enkephalin-like immunoreactivity were recorded in regions identified, respectively, as striosomes and extrastriosomal matrix. Striosomes were defined by the presence of clustered substance P-positive and dynorphin B-positive neurons and neuropil. Tests for the co-existence of enkephalin-like peptide and glutamate decarboxylase-like immunoreactivity were also made with the peroxidase-antiperoxidase method. Co-expression of substance P-like and dynorphin B-like immunoreactivities was the rule both in striosomes and in the matrix. In striosomes, substance P-like immunoreactivity was found in 96% of dynorphin B-immunoreactive neurons, and in the matrix 89% of dynorphin B-positive cells contained substance P-like immunoreactivity. Substance P/dynorphin B-positive neurons corresponded to over half (57%) of the neurons in striosomes but only 39% of the neurons in the matrix. Both in the matrix and in striosomes, about two-thirds of all neurons (63% and 65%, respectively) were identified as enkephalin-positive. Among all substance P/dynorphin B-positive medium-sized neurons, 76% also contained enkephalin-like antigen. The enkephalin-positive neurons characterized by triple peptide co-existence (enkephalin/substance P/dynorphin B) represented a mean of 63% of striosomal enkephalin-positive neurons (41% of all striosomal neurons) and 35% of matrical enkephalin-positive neurons (26% of all matrical neurons). Finally, nearly all enkephalin-positive neurons were immunoreactive for glutamate decarboxylase, and therefore probably GABAergic, but only about half the glutamate decarboxylase-positive population was enkephalin-immunoreactive. These findings suggest that neuropeptides from three distinct precursors may be co-localized in single medium-sized neurons in the striatum, and that the differential patterns of co-expression of substance P-like, dynorphin B-like, and enkephalin-like peptides may confer functional specializations upon subpopulations of GABAergic neurons giving rise to the efferent projections of the striatum. The linked expression of substance P-like and dynorphin B-like peptides in single neurons both in striosomes and matrix suggests that some regulatory mechanisms controlling peptide expression apply regardless of compartment.(ABSTRACT TRUNCATED AT 400 WORDS)
在用秋水仙碱预处理的成年猫组织中，研究了尾状核中型神经元中速激肽样和阿片样肽的表达。应用了两种方法，即连续薄切片过氧化物酶-抗过氧化物酶技术和双荧光单切片技术。主要采用过氧化物酶-抗过氧化物酶方法进行定量估计。在分别确定为纹状体小体和纹状体基质的区域记录表达P物质样、强啡肽B样和脑啡肽样免疫反应性的神经元数量。纹状体小体由聚集的P物质阳性和强啡肽B阳性神经元及神经毡的存在来定义。还用抗过氧化物酶方法检测了脑啡肽样肽与谷氨酸脱羧酶样免疫反应性的共存情况。P物质样和强啡肽B样免疫反应性的共表达在纹状体小体和基质中都是普遍现象。在纹状体小体中，96%的强啡肽B免疫反应性神经元发现有P物质样免疫反应性，在基质中，89%的强啡肽B阳性细胞含有P物质样免疫反应性。P物质/强啡肽B阳性神经元在纹状体小体中占神经元总数的一半以上（57%），但在基质中仅占39%。在基质和纹状体小体中，所有神经元中约三分之二（分别为63%和65%）被确定为脑啡肽阳性。在所有P物质/强啡肽B阳性中型神经元中，76%也含有脑啡肽样抗原。以三联肽共存（脑啡肽/P物质/强啡肽B）为特征的脑啡肽阳性神经元在纹状体小体脑啡肽阳性神经元中平均占63%（占所有纹状体小体神经元的41%），在基质脑啡肽阳性神经元中占35%（占所有基质神经元的26%）。最后，几乎所有脑啡肽阳性神经元对谷氨酸脱羧酶都有免疫反应性，因此可能是γ-氨基丁酸能的，但只有约一半的谷氨酸脱羧酶阳性细胞群是脑啡肽免疫反应性的。这些发现表明，来自三种不同前体的神经肽可能共定位于纹状体中的单个中型神经元中，并且P物质样、强啡肽B样和脑啡肽样肽的共表达差异模式可能赋予产生纹状体传出投射的γ-氨基丁酸能神经元亚群功能特化。P物质样和强啡肽B样肽在纹状体小体和基质中的单个神经元中的联合表达表明，一些控制肽表达的调节机制不受区域限制。（摘要截短至400字）
