海胆卵细胞分裂过程中巯基的研究。III. 氯化钾可溶性蛋白的巯基及其在卵裂过程中的变化。
Studies on sulfhydryl groups during cell division of sea urchin egg. III. SH groups of KC1-soluble proteins and their change during cleavage.
作者信息
SAKAI H
出版信息
J Biophys Biochem Cytol. 1960 Dec;8(3):609-15. doi: 10.1083/jcb.8.3.609.
Abstract
Sea urchin egg proteins extracted with KCl are mostly TCA-soluble and, conversely, those extracted with TCA are KCl-soluble. Both groups are water-insoluble and show fluctuations in-SH content during the division cycle. The fluctuation of the-SH groups of the KCl-soluble protein of the whole egg is due to a -SHright harpoon over left harpoon-S-S- interchange within the freely reacting groups and not within the sluggish and masked -SH groups of the protein. The -SH content of the KCl-soluble protein of the egg cortex also fluctuates in a similar way.
摘要
用氯化钾提取的海胆卵蛋白质大多可溶于三氯乙酸，相反，用三氯乙酸提取的蛋白质则可溶于氯化钾。这两组蛋白质都不溶于水，并且在分裂周期中其巯基含量会发生波动。全卵中可溶于氯化钾的蛋白质的巯基波动是由于在自由反应基团内发生了巯基（-SH）向左箭头右箭头二硫键（-S-S-）的互换，而不是在蛋白质中反应迟缓且被掩盖的巯基基团内。卵皮层中可溶于氯化钾的蛋白质的巯基含量也以类似方式波动。
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本文引用的文献
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Sulfhydryl Groups and Cell Division.
Science. 1956 Dec 28;124(3235):1292-3. doi: 10.1126/science.124.3235.1292-a.
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The Isolation and Biochemical Characterization of the Mitotic Apparatus of Dividing Cells.分裂细胞有丝分裂器的分离与生化特性分析
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J Biophys Biochem Cytol. 1958 Mar 25;4(2):157-61. doi: 10.1083/jcb.4.2.157.
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